| Assay Method Information | |
| | Glutathione S-transferase Activity Assay |
| Description: | Enzymatic activity was determined spectrophotometrically by measuring the conjugation of CDNB with GSH. The 1 ml assay mixture contained 0.5 mM CDNB, 1 mMGSH, and 100 mM potassium phosphate buffer, pH 6.5. The rate of increase in absorbance at 340 nm was measured for 5 mm at 37°C against a blank containing thereaction mixture without enzyme. |
| Affinity data for this assay | |
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