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Assay Method Information |
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| | ChEMBL_1619388 |
| Description: | Inhibition of recombinant Yersinia pestis C-terminal His6-tagged HMWP2 cysteine adenylation domain (1 to 1491 residues) expressed in Escherichia coli BL21(DE3) using 7-Methyl-6-thioguanosine as substrate preincubated for 10 mins followed by L-cysteine addition measured for 50 mins in presence of purine nucleoside phosphorylase/inorganic pyrophosphatase by spectrophotometric method |
| Affinity data for this assay | |
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