Affinity DataKd: 1.40E+3nMpH: 7.0Assay Description:The thermal shift assay (TSA) measurements were performed in a Corbett Rotor-Gene 6000 (QIAGEN Rotor-Gene Q, Sydney, Australia) instrument using the ...More data for this Ligand-Target Pair
3D Structure (crystal)Affinity DataKd: 1.40E+3nMAssay Description:Inhibition of human carbonic anhydrase 1 at pH7 and 37 degC by fluorescent thermal shift assayMore data for this Ligand-Target Pair
Affinity DataKd: 810nMAssay Description:Inhibition of human carbonic anhydrase 1 at pH7 and 37 degC by isothermal titration calorimetryMore data for this Ligand-Target Pair
Affinity DataKd: 290nMAssay Description:Inhibition of human carbonic anhydrase 1 at pH7 and 25 degC by stopped-flow kinetic inhibition assayMore data for this Ligand-Target Pair
Affinity DataKd: 1.40E+3nMAssay Description:Binding affinity to human recombinant carbonic anhydrase 1 by thermal shift assayMore data for this Ligand-Target Pair
Affinity DataKd: 780nMAssay Description:Binding affinity to human recombinant carbonic anhydrase 1 by isothermal titration calorimetry assayMore data for this Ligand-Target Pair
Affinity DataKd: 1.40E+3nMpH: 7.0Assay Description:Binding affinity to human recombinant CA1 at 37 degC and pH 7.0 by thermal shift assayMore data for this Ligand-Target Pair
Affinity DataKd: 651nMAssay Description:Binding affinity to recombinant human N-terminal His6-tagged carbonic anhydrase 1 (3 to 261 residues) expressed in Escherichia coli BL21 (DE3) assess...More data for this Ligand-Target Pair
Affinity DataKd: 1.40E+3nMAssay Description:Binding affinity to recombinant human N-terminal His6-tagged carbonic anhydrase 1 (3 to 261 residues) expressed in Escherichia coli BL21 (DE3) in pre...More data for this Ligand-Target Pair
Affinity DataKd: 1.10E+3nMAssay Description:Binding affinity to recombinant human carbonic anhydrase 1 expressed in Escherichia coli expression system assessed as kinetic dissociation constant ...More data for this Ligand-Target Pair
Affinity DataKd: 2.40E+3nMAssay Description:Binding affinity to recombinant human carbonic anhydrase 1 expressed in Escherichia coli expression system assessed as observed dissociation constant...More data for this Ligand-Target Pair
Affinity DataKd: 1.40E+3nMAssay Description:Binding affinity to human recombinant carbonic anhydrase 1 expressed in Escherichia coli BL21 (DE3) by thermal shift assayMore data for this Ligand-Target Pair
Affinity DataIC50: 5.80nMAssay Description:Inhibition of human erythrocyte carbonic anhydrase-1More data for this Ligand-Target Pair
Affinity DataIC50: 5.80nMAssay Description:Inhibition of carbonic anhydrase-1 in human erythrocyte membranes assessed as reduction in H+ release using CO2 as substrate by bromine thymol blue i...More data for this Ligand-Target Pair
Affinity DataIC50: 12nMAssay Description:Compounds which are CAIs are well known in the art, see for example, Pastorekova et al, Journal of Enzyme Inhibition and Medicinal Chemistry, 19(3), ...More data for this Ligand-Target Pair
Affinity DataIC50: 32nMAssay Description:Inhibition of human CA1 using 4-nitrophenylacetate as substrate by spectrophotometrical analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 48nMAssay Description:Inhibition of human recombinant carbonic anhydrase 1 preincubated for 15 mins by stopped flow CO2 hydration methodMore data for this Ligand-Target Pair
Affinity DataIC50: 161nMAssay Description:Inhibition of full length carbonic anhydrase-1 in human erythrocytesMore data for this Ligand-Target Pair
Affinity DataIC50: 197nMAssay Description:Inhibition of human CA1 by stopped-flow assayMore data for this Ligand-Target Pair
Affinity DataIC50: 197nMAssay Description:Inhibition of human CA1More data for this Ligand-Target Pair
Affinity DataIC50: 250nMAssay Description:Inhibitory activity of compound against human carbonic anhydrase IMore data for this Ligand-Target Pair
Affinity DataIC50: 250nMAssay Description:Inhibition of human recombinant CA1 incubated for 15 mins prior to testing by phenol red based stopped-flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataIC50: 295nMAssay Description:Inhibition of CA-1 (unknown origin) after 15 mins by CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataIC50: 299nMAssay Description:Inhibition of human recombinant carbonic anhydrase 1 pretreated for 15 mins by stopped flow CO2 hydrase assayMore data for this Ligand-Target Pair
Affinity DataIC50: 300nMAssay Description:Inhibition of recombinant human carbonic anhydrase 1 using 4-nitrophenylacetate as substrateMore data for this Ligand-Target Pair
Affinity DataIC50: 300nMAssay Description:Inhibition of recombinant human CA1 using 4-nitrophenylacetate as substrate by esterase assayMore data for this Ligand-Target Pair
Affinity DataIC50: 300nMAssay Description:Inhibition of human CA1 using 4-nitrophenylacetate as substrate incubated for 10 mins prior to testing by spectrophotometry based esterase assayMore data for this Ligand-Target Pair
Affinity DataIC50: 330nMAssay Description:Inhibition of human carbonic anhydrase 1 assessed as 4-nitrophenyl phosphate hydrolysisMore data for this Ligand-Target Pair
Affinity DataIC50: 350nMpH: 7.4 T: 2°CAssay Description:An SX.18MV-R Applied Photophysics (Oxford, UK) stopped-flow instrument has been used to assay the catalytic/inhibition of various CA isozymes as repo...More data for this Ligand-Target Pair
Affinity DataIC50: 361nMAssay Description:Inhibition of human erythrocyte CA1 using 4-nitrophenyl acetate as substrate by spectrophotometryMore data for this Ligand-Target Pair
Affinity DataIC50: 510nMAssay Description:Inhibition of recombinant human carbonic anhydrase 1 after 15 mins by stopped flow CO2 hydration assayMore data for this Ligand-Target Pair
Affinity DataIC50: 900nMAssay Description:Inhibition of carbonic anhydrase 1 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 1.21E+3nMAssay Description:Inhibition of human carbonic anhydrase 1 assessed as 4-nitrophenyl acetate hydrolysisMore data for this Ligand-Target Pair
Affinity DataIC50: 2.90E+3nMAssay Description:Inhibition of esterase activity of human carbonic anhydrase 1 by CO2 hydration methodMore data for this Ligand-Target Pair
Affinity DataIC50: 3.10E+3nMAssay Description:Inhibition of human carbonic anhydrase 1 hydratase activity by spectrophotometryMore data for this Ligand-Target Pair
Affinity DataIC50: 3.30E+3nMAssay Description:The Ki values were determined as described in the literature [Landolfi et al., J. Pharmacol. Toxicol. Methods, 38:169-172; B lb l et al., J. Enzyme I...More data for this Ligand-Target Pair
Affinity DataIC50: 3.30E+3nMAssay Description:Carbonic anhydrase activity was assayed by following the hydration of CO2 according to the method described by Wilbur and Anderson [Wilbur et al., J....More data for this Ligand-Target Pair
Affinity DataIC50: 3.50E+3nMpH: 7.4Assay Description:Carbonic anhydrase activity was assayed by following the change in absorbance at 348 nm of 4-nitrophenylacetate (NPA) to 4-nitrophenylate ion over a ...More data for this Ligand-Target Pair
Affinity DataIC50: 3.60E+3nMAssay Description:The carbonic anhydrase activity was assayed by following the hydration of CO2 according to the method described by Wilbur and Anderson [Wilbur et al....More data for this Ligand-Target Pair
Affinity DataIC50: 3.60E+3nMAssay Description:Inhibition of hydratase-activity of CA1 from human erythrocytes by CO2-hydration methodMore data for this Ligand-Target Pair
Affinity DataIC50: 3.75E+3nMAssay Description:Inhibition of CO2-hydratase activity of human carbonic anhydrase 1 by CO2 hydration methodMore data for this Ligand-Target Pair
Affinity DataIC50: 3.80E+3nMAssay Description:Inhibition of human carbonic anhydrase 1 esterase activity by spectrophotometryMore data for this Ligand-Target Pair
Affinity DataIC50: 4.20E+3nMAssay Description:Carbonic anhydrase (CA) activity was assayed by following the hydration of CO2 according to the method described by Wilbur and Anderson [Wilbur et al...More data for this Ligand-Target Pair
Affinity DataIC50: 4.60E+3nMpH: 7.4Assay Description:Carbonic anhydrase activity was assayed by following the change in absorbance at 348 nm of 4-nitrophenylacetate (NPA) to 4-nitrophenylate ion over a ...More data for this Ligand-Target Pair
Affinity DataIC50: 5.20E+3nMAssay Description:The method for determination of the Ki values is described elsewhere [Landolfi et al., J. Pharmacol. Toxicol. Methods, 38:169-172; B lb l et al., J. ...More data for this Ligand-Target Pair
Affinity DataIC50: 5.20E+3nMAssay Description:Inhibition of CA1 from human erythrocytes by Lineweaver-Burke analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 5.55E+3nMAssay Description:The method for determination of Ki values is described elsewhere [Landolfi et al., J. Pharmacol. Toxicol. Methods, 38:169-172; B lb l et al., J. Enzy...More data for this Ligand-Target Pair
Affinity DataIC50: 5.90E+3nMpH: 7.4 T: 2°CAssay Description:Carbonic anhydrase activity was assayed by following the change in absorbance at 348 nm of 4-nitrophenylacetate (NPA) to 4-nitrophenylate ion over a ...More data for this Ligand-Target Pair
Affinity DataIC50: 6.10E+3nMpH: 7.4Assay Description:The carbonic anhydrase activity was assayed by following the change in absorbance at 348 nm of the 4-nitrophenylacetate (NPA) to the 4-nitrophenylate...More data for this Ligand-Target Pair
Affinity DataIC50: 6.10E+3nMAssay Description:Inhibition of esterase-activity of CA1 from human erythrocytes by spectrophotometryMore data for this Ligand-Target Pair
