Affinity DataKi: 6nM ΔG°: -11.7kcal/mole IC50: 32nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 9nM ΔG°: -11.4kcal/mole IC50: 51nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 11nM ΔG°: -11.3kcal/mole IC50: 60nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 13nM ΔG°: -11.2kcal/mole IC50: 73nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 19nM ΔG°: -10.9kcal/mole IC50: 107nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 20nM ΔG°: -10.9kcal/mole IC50: 112nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 26nM ΔG°: -10.8kcal/mole IC50: 146nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 28nM ΔG°: -10.7kcal/mole IC50: 154nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 40nM ΔG°: -10.5kcal/mole IC50: 222nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 41nM ΔG°: -10.5kcal/mole IC50: 231nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
3D Structure (crystal)
Affinity DataKi: 44nM ΔG°: -10.4kcal/mole IC50: 245nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 46nM ΔG°: -10.4kcal/mole IC50: 255nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 49nM ΔG°: -10.4kcal/mole IC50: 271nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 53nM ΔG°: -10.3kcal/mole IC50: 297nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 58nM ΔG°: -10.3kcal/mole IC50: 325nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 69nMAssay Description:Inhibitory activity against cell free dihydrofolate redutase (DHFR) from Escherichia coliMore data for this Ligand-Target Pair
Affinity DataKi: 81nM ΔG°: -10.1kcal/mole IC50: 454nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 104nM ΔG°: -9.90kcal/mole IC50: 580nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 111nM ΔG°: -9.86kcal/mole IC50: 619nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 120nMAssay Description:Inhibition of West Nile virus NS2B (47 residues)-NS3 (184 residues) protease expressed in Escherichia coli BL21 (DE3) preincubated for 15 mins follow...More data for this Ligand-Target Pair
Affinity DataKi: 130nMAssay Description:Inhibition of West Nile virus NS2B-NS3 proteaseMore data for this Ligand-Target Pair
Affinity DataKi: 130nMAssay Description:Inhibitory activity against cell free dihydrofolate redutase (DHFR) from Mycobacterium lufuMore data for this Ligand-Target Pair
Affinity DataKi: 160nM ΔG°: -9.64kcal/mole IC50: 891nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 343nMAssay Description:Competitive inhibition of C-terminal His-tagged WNV NSB2 (52 to 96 amino acids)-NS3 (1 to 184 amino acids) protease expressed in Escherichia coli BL2...More data for this Ligand-Target Pair
Affinity DataKi: 400nMAssay Description:Inhibitory activity against cell free dihydrofolate redutase (DHFR) from Escherichia coliMore data for this Ligand-Target Pair
Affinity DataKi: 820nMAssay Description:Inhibition of WNV NS2B-NS3 protease expressed in Escherichia coli BL21 lambda (DE3) cells using Abz-Gly-Leu-Lys-Arg-Gly-Gly-3-(NO2)Tyr as substrate p...More data for this Ligand-Target Pair
Affinity DataKi: 1.00E+3nMAssay Description:Inhibition of West Nile virus NS2B-NS3 protease expressed in Escherichia coli BL21 lambda (DE3) cells using Abz-Gly-Leu-Lys-Arg-Gly-Gly-3-(NO2)Tyr as...More data for this Ligand-Target Pair
Affinity DataKi: 1.30E+3nMAssay Description:Competitive inhibition of WNV NS2B-NS3 protease expressed in Escherichia coli BL21 lambda (DE3) cells using Abz-Gly-Leu-Lys-Arg-Gly-Gly-3-(NO2)Tyr as...More data for this Ligand-Target Pair
Affinity DataKi: 2.00E+3nMAssay Description:Inhibition of West Nile virus NS2B-NS3 proteaseMore data for this Ligand-Target Pair
Affinity DataKi: 2.07E+3nM ΔG°: -8.06kcal/mole IC50: 1.16E+4nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 3.06E+3nMAssay Description:Inhibitory activity against cell free dihydrofolate redutase (DHFR) from Escherichia coliMore data for this Ligand-Target Pair
Affinity DataKi: 4.47E+3nMAssay Description:Inhibitory activity against cell free dihydrofolate redutase (DHFR) from Mycobacterium lufuMore data for this Ligand-Target Pair
Affinity DataKi: 4.60E+3nM ΔG°: -7.57kcal/mole IC50: 2.57E+4nMpH: 9.5 T: 2°CAssay Description:Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis wa...More data for this Ligand-Target Pair
Affinity DataKi: 4.90E+3nMAssay Description:Inhibition of West Nile virus NS2B-NS3 protease preincubated for 1 hr followed by Pyr-RTKR-AMC addition and measured after 30 mins by fluorescence me...More data for this Ligand-Target Pair
Affinity DataKi: 7.40E+3nMAssay Description:Inhibition of C-terminal His6-tagged West Nile virus NS2B (52 to 96 residues)-G4SG4-NS3 (1 to 184 residues) expressed in Escherichia coli BL21(DE3) c...More data for this Ligand-Target Pair
Affinity DataKi: 8.00E+3nMAssay Description:Inhibition of West Nile virus NS2B-NS3 protease preincubated for 10 mins followed by Np-KKR-pNA addition and measured every 5 secs for 2 mins by fluo...More data for this Ligand-Target Pair
Affinity DataKi: 1.20E+4nMAssay Description:Inhibition of West Nile virus NS2B-NS3 protease expressed in Escherichia coli BL21 lambda (DE3) cells using Abz-Gly-Leu-Lys-Arg-Gly-Gly-3-(NO2)Tyr as...More data for this Ligand-Target Pair
Affinity DataKi: 1.30E+4nM ΔG°: -6.62kcal/molepH: 8.5 T: 2°CAssay Description:All proteases were assayed by monitoring the cleavage of peptide substrate with fluorescent reporter at room temperature with a microtiter plate spec...More data for this Ligand-Target Pair
Affinity DataKi: 1.60E+4nM ΔG°: -6.49kcal/molepH: 8.5 T: 2°CAssay Description:All proteases were assayed by monitoring the cleavage of peptide substrate with fluorescent reporter at room temperature with a microtiter plate spec...More data for this Ligand-Target Pair
Affinity DataKi: 2.55E+4nMAssay Description:Inhibition of C-terminal His6-tagged West Nile virus NS2B (52 to 96 residues)-G4SG4-NS3 (1 to 184 residues) expressed in Escherichia coli BL21(DE3) c...More data for this Ligand-Target Pair
Affinity DataKi: 3.50E+4nM ΔG°: -6.03kcal/molepH: 8.5 T: 2°CAssay Description:All proteases were assayed by monitoring the cleavage of peptide substrate with fluorescent reporter at room temperature with a microtiter plate spec...More data for this Ligand-Target Pair
Affinity DataKi: 4.90E+4nMAssay Description:Inhibition of West Nile virus serotype 2 NS2B-NS3 protease by homogeneous fluorimetric assayMore data for this Ligand-Target Pair
Affinity DataKi: 9.25E+4nMAssay Description:Inhibitory activity against cell free dihydrofolate redutase (DHFR) from Mycobacterium lufuMore data for this Ligand-Target Pair
Affinity DataKi: 9.25E+4nMAssay Description:Inhibition of West Nile virus NS2B-NS3 protease preincubated for 15 mins followed by Abz-Gly-Leu-Lys-Arg-Gly-Gly-3-(NO2)Tyr addition and measured for...More data for this Ligand-Target Pair
Affinity DataKi: 3.37E+5nM ΔG°: -4.70kcal/molepH: 8.5 T: 2°CAssay Description:All proteases were assayed by monitoring the cleavage of peptide substrate with fluorescent reporter at room temperature with a microtiter plate spec...More data for this Ligand-Target Pair
Affinity DataKi: 5.00E+5nMAssay Description:Inhibitory activity against cell free dihydrofolate redutase (DHFR) from Escherichia coliMore data for this Ligand-Target Pair
Affinity DataKi: 1.09E+6nM ΔG°: -4.01kcal/molepH: 8.5 T: 2°CAssay Description:All proteases were assayed by monitoring the cleavage of peptide substrate with fluorescent reporter at room temperature with a microtiter plate spec...More data for this Ligand-Target Pair
