Affinity DataEC50: 80nMAssay Description:Inhibition of EGF-stimulated ERK5 autophosphorylation in human HeLa cells preincubated with compound for 1 hrs followed by EGF stimulation for 17 min...More data for this Ligand-Target Pair
Affinity DataIC50: 82nMAssay Description:Inhibition of N-terminal 6His-tagged human ERK5 expressed in baculovirus infected Sf21 cells co-expressing HA-tagged human MEK5-DD using ARKKRRHPSGPP...More data for this Ligand-Target Pair
Affinity DataIC50: 162nM EC50: 90nMpH: 7.5Assay Description:Kinase activity was determined in an assay volume of 40 μl in kinase buffer (50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 1 mM 2-mercaptoethanol) contai...More data for this Ligand-Target Pair

3D Structure (crystal)