Reaction Details  Report a problem with these data
Cell Reactant:
HyHEL-5 Mutant(E50D)
Syringe Reactant:
Lysozyme (hen egg)
Meas. Tech.:
ITC and sedimentation equilibrium
Entry Date.:
12/13/00
ΔG°:
-44.1±0.42 (kJ/mole)
pH:
8±n/a
Temperature:
298.15±n/a (K)
ΔH° :
-78.54±2.52 (kJ/mole)
ΔHobs :
-78.54±n/a (kJ/mole)
Corrected for ΔHioniz:
not known
ΔCp :
-1.11±0.164 (kJ/mole)
Stoich. Param.:
1
ΔS° :
-0.116±0.006 (kJ/mole-K)
Citation
 Wibbenmeyer, JA; Schuck, P; Smith-Gill, SJ; Willson, RC Salt links dominate affinity of antibody HyHEL-5 for lysozyme through enthalpic contributions. J Biol Chem 274:26838-42 (1999) [PubMed]  Article
Cell React
Source:
Site-directed mutagenesis of recombinant HyHEL-5 Fab
Purity:
95%
Prep. Method:
Refolded protein was purified by Q Sepharose and nickel-charged chelating Sepharose Fast Flow Chromatography
Name:
HyHEL-5 Mutant(E50D)
Synonyms:
HyHEL-5 Fab mutant (E50D)
Type:
Antibody
Mol. Mass.:
22997.82
Organism:
Mus musculus (mouse)
Description:
mutant of murine monoclonal antibody HyHEL-5 to hen egg lysozyme
Residue:
215
Sequence:
EVQLQQSGAELMKPGASVKISCKASGYTFSDYWIEWVKQRPGHGLEWIGDILPGSGSTNYHERFKGKATFTADTSSSTAYMQLNSLTSEDSGVYYCLHGNYDFDGWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKILD
  
Syringe React
Source:
Worthington Biologicals
Name:
Lysozyme C
Synonyms:
FvD1.3(VLW92A) | FvD1.3(VLW92D) | FvD1.3(VLW92F) | FvD1.3(VLW92H) | FvD1.3(VLW92S) | FvD1.3(VLW92V) | FvD1.3(VLW92wt) | LYSC_CHICK | LYZ | Lysozyme (hen egg) | hen egg lysozyme (HEL)
Type:
Enzyme
Mol. Mass.:
16248.07
Organism:
Gallus gallus (chicken)
Description:
P00698
Residue:
147
Sequence:
MRSLLILVLCFLPLAALGKVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL