Reaction Details Report a problem with these data
Target
Nitric oxide synthase, endothelial
Ligand
BDBM21972
Substrate
BDBM21959
Meas. Tech.
NOS Enzyme Inhibition Assay
pH
7.4±n/a
Temperature
303.15±n/a K
IC50
1342000±n/a nM
Km
1700±n/a nM
Citation
 Erdal, EP; Martásek, P; Roman, LJ; Silverman, RB Hydroxyethylene isosteres of selective neuronal nitric oxide synthase inhibitors. Bioorg Med Chem 15:6096-108 (2007) [PubMed]  Article 
Target
Name:
Nitric oxide synthase, endothelial
Synonyms:
Constitutive NOS | Endothelial NOS | Endothelial nitric oxide synthase | Endothelial nitric-oxide synthase (eNOS) | NOS type III | NOS3 | NOS3_BOVIN | Nitric oxide synthase, endothelial (eNOS) | Nitric-oxide synthase, endothelial | cNOS
Type:
Enzyme
Mol. Mass.:
133292.26
Organism:
Bos taurus (bovine)
Description:
Recombinant eNOS overexpressed in E. coli was used in enzyme assays.
Residue:
1205
Sequence:
MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSRAPAPATPHAPDHSPAPNSPTLTRPPEGPKFPRVKNWELGSITYDTLCAQSQQDGPCTPRCCLGSLVLPRKLQTRPSPGPPPAEQLLSQARDFINQYYSSIKRSGSQAHEERLQEVEAEVASTGTYHLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCSSAQEMFTYICNHIKYATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFSAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDNEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYILSPAFRYQPDPWKGSATKGAGITRKKTFKEVANAVKISASLMGTLMAKRVKATILYASETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHEALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAKAAFQASCETFCVGEEAKAAAQDIFSPKRSWKRQRYRLSTQAEGLQLLPGLIHVHRRKMFQATVLSVENLQSSKSTRATILVRLDTAGQEGLQYQPGDHIGICPPNRPGLVEALLSRVEDPPPPTESVAVEQLEKGSPGGPPPSWVRDPRLPPCTLRQALTFFLDITSPPSPRLLRLLSTLAEEPSEQQELETLSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPNAHPGEVHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKTGDPVPCFIRGAPSFRLPPDPYVPCILVGPGTGIAPFRGFWQERLHDIESKGLQPAPMTLVFGCRCSQLDHLYRDEVQDAQERGVFGRVLTAFSREPDSPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATSVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERHLRGAVPWAFDPPGPDTPGP
  
Inhibitor
Name:
BDBM21972
Synonyms:
3-[(4S,6R)-4,8-diamino-6-hydroxyoctyl]-1-nitroguanidine | Hydroxyethylene derivative, 11
Type:
n/a
Emp. Form.:
C9H22N6O3
Mol. Mass.:
262.3094
SMILES:
NCC[C@@H](O)C[C@@H](N)CCCN=C(N)N[N+]([O-])=O |w:11.10|
Structure:
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Substrate
Name:
BDBM21959
Synonyms:
(2S)-2-amino-5-carbamimidamidopentanoic acid | ARGININE | CHEMBL1485 | L-arginine
Type:
Amino Acid
Emp. Form.:
C6H14N4O2
Mol. Mass.:
174.201
SMILES:
[#7]-[#6@@H](-[#6]-[#6]-[#6]\[#7]=[#6](/[#7])-[#7])-[#6](-[#8])=O
Structure:
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