Target

Ligand

Substrate

Meas. Tech.

FRET-Based RNase H Assay

pH

8±n/a

Temperature

293.15±n/a K

Citation

 Wendeler, M; Lee, HF; Bermingham, A; Miller, JT; Chertov, O; Bona, MK; Baichoo, NS; Ehteshami, M; Beutler, J; O'Keefe, BR; Götte, M; Kvaratskhelia, M; Le Grice, S Vinylogous ureas as a novel class of inhibitors of reverse transcriptase-associated ribonuclease H activity. ACS Chem Biol 3:635-44 (2008) [PubMed]  Article Copy BDB DOI

More Info.:

Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method

Name:

Ribonuclease HI

Synonyms:

RNH_ECOLI | RNase H | Ribonuclease H | Ribonuclease HI | dasF | herA | rnh | rnhA | sdrA

Type:

Enzyme

Mol. Mass.:

17601.33

Organism:

Escherichia coli (strain K12)

Description:

n/a

Residue:

155

Sequence:

MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTNNRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVDLWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV

Blast this sequence in BindingDB or PDB

  
Blast E-value cutoff:

Name:

BDBM33963

Synonyms:

vinylogous urea, NSC727448

Type:

Small organic molecule

Emp. Form.:

C12H12N2O3S

Mol. Mass.:

264.3

SMILES:

Cc1sc(NC(=O)c2ccco2)c(C(N)=O)c1C

Structure:

Search PDB for entries with ligand similarity:

Similarity to this molecule at least:

Name:

RNA/DNA hybrid

Synonyms:

n/a

Type:

fluorescein-labeled RNA annealed to DNA

Mol. Mass.:

2134.40

Organism:

n/a

Description:

It is a 18-nucleotide 3-fluorescein-labeled RNA annealed to a complementary 18-nucleotide 5-Dabcyl-modified DNA.

Residue:

23

Sequence:

GACGAGCCGGGAGCFLRESCEIN