| Assay Method Information | |
| | NF-keppaB Inhibitory Activity Evaluation Test |
| Description: | A549/NF-κB-luc cells (available from Panomics, Cat No. LR0051) were prepared at 375,000 cells/mL in the culture medium. The 375,000 cells/mL A549/NF-κB-luc cell suspension was seeded at 80 μL each on a white 96-well plate (available from Greiner, Cat No. 655073). One hour after seeding the cells, 10 μL of 2% DMSO solution (control) and each compound solution in each concentration were added to each well of the 96-well plate. One hour after the addition of each compound solution, 10 ng/mL of the TNF-α solution (10 μL) was added to each well of the 96-well plate. Three hours after the addition of 10 ng/mL of the TNF-α solution, 100 μL of Steady-Glo reagent (available from Promega, Cat No. E2510) was added to each well of the 96-well plate. After 30 to 90 minutes of the addition of the Steady-Glo reagent, the luminescence intensity of each well was detected by EnVision multilabel counter (available from PerkinElmer). |
| Affinity data for this assay | |
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