| Assay Method Information | |
| | Fluorescence Resonance Energy Transfer Assay (FRET) |
| Description: | The enzymatic reaction started by the addition fluorogenic peptide substrate, MAPKKide to the buffer containing LF and inhibitor compound. Cleavage of the substrate by LF released the fluorophore and full fluorescence was restored. Fluorescence intensity (Ex: 320 nm, Em: 420 nm) was monitored for 15 min at room temperature and the Ki values were calculated using the program BatchKi (BioKin Ltd., Pullman, WA). |
| Affinity data for this assay | |
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