| Assay Method Information | |
| | In Vitro Assay-SREBP-2 |
| Description: | 10,000 cells/well of the PathHunter U2OS SREBP-2 Nuclear Translocation Cell Line are plated in quadruplicate on 384 well plates in 20 μL of AssayComplete cell plating 5 reagent (CP5-high serum) overnight at 37° C./5% CO2. A dilution series of test compound is added to cells, incubated for 1 hour at 37° C./5% CO2, and U18666A (a known SREBP agonist) is added to a final concentration of 10 μM. The plate is incubated overnight (16 hours) at 37° C./5% CO2. PathHunter Flash detection mix is added to the wells, incubated one hour in the dark, and the plate read on an Envision luminometer. |
| Affinity data for this assay | |
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