| Assay Method Information | |
| | PDE4B Inhibitory Activity Assay |
| Description: | In order to evaluate the PDE4B inhibitory activity of the compounds according to the present invention, the following experiment was performed using LANCE Ultra cAMP assay kit (Perkin Elmer, U.S.A).Particularly, 5 μl of the 3 nM cAMP dissolved reaction buffer was added to each reaction well (LANCE Ultra cAMP assay kit). Then, each example compound dilution solution (2.5 μl) and PDE4B enzyme (0.1 ng/well, BPS Biosciences, San Diego, U.S.A) were added to the well, followed by incubation for 1 hour at 37° C. Next, 5 μl of ULight-anti-cAMP detection reactant supplemented with 5 μl of Eu-cAMP tracer and 1 mM IBMX (LANCE Ultra cAMP assay kit) was added thereto, followed by incubation for 1 hour at 37° C. Upon completion of the incubation, the signals excitated at 340 nm and emitted at 665 nm from the 384 micro well-plate were recorded using EnVision Multilabel Reader (Perkin Elmer, U.S.A.). |
| Affinity data for this assay | |
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