| Assay Method Information | |
| | AlphaLISA assay |
| Description: | According to the specification, test buffer was used to dilute the enzyme complex, methyl donor S-adenosylmethionine (SAM) (Sigma, Art. No. A7007), protease inhibitor (sinefungin) (Sigma, Art. No. S8559), biotinylated peptide substrate (AnaSpec, Art. No. 64440), 2.5 microliter of 4-fold enzyme complex (BPS, Art. No. 51004), 2.5 microliter of 4-fold test inhibitor buffer, 5 microliter of biotinylated histone (H3) and 2-fold methyl donor S-adenosylmethionine were added to a 384-well plate and incubated at room temperature. Finally, 15 microliter of detection solution mixture was added under weak light and incubated at room temperature for 60 minutes, and the value was recorded. |
| Affinity data for this assay | |
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