Assay Method Information | |
| Kinase Inhibition Assay |
Description: | Kinase activity was assayed in reaction buffer containing substrate, enzyme, and inhibitor in the presence of 0.17 mM ATP/[gamma-32P] ATP. 32P incorporation was measured using a scintillation counter. The IC50 is the inhibitor concentration, which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from 32P labeled ATP to the substrate. |
Affinity data for this assay | |
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