| Assay Method Information | |
| | In Vitro GST Activity Assay |
| Description: | The PGDS glutathione-S-transferase (GST) activity was measured by using MonoChloroBimane (MCB) as a chromogenic substrate. The assay was run at 384-well format in reaction buffer containing enzyme and test compound. Then MCB and GSH were added for further incubation. After reaction was stopped, the fluorescence was measured (excitation: 390 nm; emission: 478 nm). |
| Affinity data for this assay | |
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