| Assay Method Information | |
| | AE Enzyme Inhibition Assay |
| Description: | AE activity in the induction medium was measured with the substrate Cbz-Ala-Ala-Asn-AMC. In a black 96-well microtiter plate, aza-peptidyl inhibitors were added to the reaction buffer containing the activated enzyme, and allowed to incubate at room temperature for 20 min. Then, substrate Cbz- Ala-Ala-Asn-AMC was added and the reaction allowed proceeding for 20 min. Plotting the relative fluorescence units (RFU)/min against the inhibitor concentration (uM) permitted calculation of the IC50 value. |
| Affinity data for this assay | |
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