| Assay Method Information | |
| | Phosphatase Activity Assay |
| Description: | PTP activity was measured in a black 96-well plate. Reaction was initiated by addition of the substrate DiFMUP to enzyme mixtures containing test compounds. The fluorescence signal was measured at 355 nm (excitation) and 460 nm (emission) with plate reader. For IC50 determination, eight concentrations of compounds at 1/3 dilution (~0.5 log) were tested. Each experiment was performed in duplicate, and IC50 data were derived from at least 4 independent experiments. The curvefitting program Prism 4 (GraphPad Software) was used to calculate the IC50 value. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |