| Assay Method Information | |
| | Aurora Kinase Assay |
| Description: | Compounds were diluted into assay buffer containing Aurora kinase and FAM-PKAtide. The kinase reaction was initiated by adding ATP in assay buffer. To detect phosphorylated PKAtide, the kinase reaction was combined with Progressive Binding Solution (Molecular Devices). The mixture was incubated, and then the plate was scanned on an Analyst AD with excitation at 485 nm and emission at 530 nm. Relative enzymatic activity values were plotted as a function of the logarithm of compound concentration and IC50 values were generated in GraphPad Prism software using a sigmoidal dose-response curve-fit. |
| Affinity data for this assay | |
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