Assay Method Information | |
| Fluorescence Peptide Cleavage Assay |
Description: | Fluorescence peptide cleavage assay was performed in a 96-well plate. Each reaction contained fluorogenic peptide substrate, LF, and small-molecule inhibitor. Substrate was cleaved between Pro (8)-Ile (9) with an increase in fluorescence. The reaction was examined by using a fluorescent plate reader at excitation and emission wavelength at 380 and 450 nm. |
Affinity data for this assay | |
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