| Assay Method Information | |
| | Confirmation Cuvette-Based Assay for Inhibitors of AmpC Beta-Lactamase (assay with detergent) |
| Description: | NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: MH079825-01 Assay Provider: Shoichet, Brian K. This aggregation profiling approach exploits the sensitivity of aggregate formation to detergent. Inhibition of b-lactamase is measured in the presence and absence of 0.01% Triton X-100 (Feng 2007). This particular assay is a confirmation of previous qHTS (Inglese, 2006), Pubchem AID 584, assay with presence of 0.01% Triton X-100. For a related assay without detergent, see AID 585. Compounds that inhibit only in the absence of detergent are considered likely promiscuous aggregators. This confirmation assay is a cuvette-based screen of an oxadiazole carbamate series which is an expansion around docking hit Pubchem SID 4244870. |
| Affinity data for this assay | |
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