| Assay Method Information | |
| | Detection of SPR Binding Assays (Surface Plasmon Resonance Binding Assay) of TRIM21 Compounds |
| Description: | Preparation of Running BufferA protein fixation buffer included 7.6 mM NaH2OP4, 12.4 mM Na2HOP4, 150.0 mM NaCl, and 0.1% of Tween 20, and adjusted to pH 7.0. A running buffer A included 7.6 mM NaH2OP4, 12.4 mM Na2HOP4, 150.0 mM NaCl, and 0.1% Tween 20, and adjusted to pH 7.0. A running buffer B included 7.6 mM NaH2OP4, 12.4 mM Na2HOP4, 150.0 mM NaCl, 0.1% of Tween 20 and 1.00% of DMSO, and adjusted to pH 7.0. The protein fixation buffer, the running buffer A and the running buffer B were prepared and filtered through a 0.22 m membrane prior to use. TRIM21 Protein FixationThe running buffer A was used as the protein fixation buffer. The protein fixation buffer and the TRIM21 protein were mixed to prepare a 40.0 g/mL mixed solution. The 40.0 g/mL mixed solution was injected at flow rate of 5.0 μL/min for 600 s, and the TRIM21 protein was captured on the CM5 chip through Strep tactin XT.A final fixation amount of the TRIM21 protein was about 2200.00 RU. |
| Affinity data for this assay | |
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