| Assay Method Information | |
| | Dose Response of primary screening hit compounds for Identification of VLA-4 Allosteric Modulators |
| Description: | University of New Mexico Assay Overview: Assay Support Project Title:HTS for Identification of VLA-4 Allosteric Modulators 1 R01 HL081062-01 PI:Larry Sklar PhD and Alex Chigaev PhD Assay Implementation: Peter Simons PhD, Susan Young MS, Yang Wu PhD, Terry Foutz, Stephanie Sedillo, Anna Waller PhD, Mark Carter MS Assay Background and Significance: We have developed a novel ligand induced binding site (LIBS) mAb assay for integrin HTS using phycoerythrin (PE) labeled HUTS-21 mAb. The novel assay is a result of an R01 (HL081062) which has taken advantage of compounds identified through an earlier project X01MH077638 (entitled "MLSCN Assay for Allosteric Regulators of the VLA-4 Integrin") that screened a portion of the MLSMR for allosteric regulators of VLA-4. This approach uses an improved assay to search for novel integrin ligands that block the binding of traditional ligands and do not in themselves induce the LIBS mAb binding site. Molecules of this type are unknown for VLA-4. A |
| Affinity data for this assay | |
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