Assay Method Information | |
| ChEMBL_1711161 (CHEMBL4121210) |
Description: | Inhibition of N-terminal His6-tagged human liver PHGDH expressed in Escherichia coli Rosetta (DE3)pLysS using 3-phosphoglycerate as substrate after 20 mins in presence of NAD+ by resazurin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay |
Affinity data for this assay | |
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