| Assay Method Information | |
| | ATX Inhibitory Selectivity Test |
| Description: | Inhibitory activities of hit compounds towards ENPP1-5 were calculated using pNP-TMP, and the inhibitory activities of hit compounds towards ENPP6 and 7 were calculated using pNPPC. The final concentration of these two absorption-based substrates was 100uM. Assay buffer (ENPP buffer, 37uL) and 500uM PNP-TMP or pNPPC (20uL) were dispensed into the 96-well microplate. 30uM samples (33uL) were added, followed by ENPP1-7 solution (10uL) into each well. Each well contained a total of 100uL. The plate was incubated for 2 hours and aborbance (405nm) was measured with a microplate reader. |
| Affinity data for this assay | |
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