| Assay Method Information | |
| | Inhibition Activity Assay |
| Description: | The capacities of compounds of the present invention to inhibit the activity of lysine gingipain (Kgp) were measured in a fluorogenic assay similar to those described by Barret (Biochemical Journal. 1980, 187(3), 909) using 10 M Z-His-Glu-Lys-MCA as a fluorogenic substrate at 37 ° C. By testing a range of concentrations for each compound, the concentration required to inhibit the activity of lysine gingipain by 500(the IC50 ) was determined. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |