Assay Method Information

Assay Name:  MCT1-Mediated Lactate Transport in BT20 Breast Cancer Cells
Description:  MCT1 activity may be measured using BT-20 breast cancer cells that express high native levels of MCT1, but do not express MCT4 and are known to those with skill in the art. Preparation of BCECF loaded cells are as described for Assay 1. Lactate transport assay is as described for Assay 1, except 10 mM L-lactate (rather than 50 mM) is added.
Affinity data for this assay
 

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