| Assay Method Information | |
| | Cell-Based Functional Assay (5-HT7) |
| Description: | CHO cells were transfected with an expression vector encoding rat 5-HT7 gene and CRE-Luc reporter system. Colonies were isolated and screened with 1000 nM 5-HTfor the induction of luciferase reporter gene. The clone demonstrating highest 5-HT induced reporter gene was used in the evaluation of the known and novel compounds. Increasing concentrations of the compound were incubated with or without 1000 nM 5-HT for antagonist or agonist mode respectively for 5 h in 96 well plate. After incubation, cells were harvested, lysed and reporter gene activity was measured. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |