| Assay Method Information | |
| | RSV Antiviral Assay (RSV) |
| Description: | The HeLa-derived cells containing the stable RSV replicon were cultured in DMEM containing 4500 mg/L D-glucose, L-glutamine, and 110 mg/L sodium pyruvate. The medium was further supplemented with 10% (v/v) FBS (Mediatech), 1% (v/v) penicillin/streptomycin (Mediatech), and 10 μg/mL of Blasticidin (BSD) (Invivogen). Cells were maintained at 37° C. in a humidified 5% CO2 atmosphere. On the first day, 5000 RSV replicon cells per well were plated in a 96-well plate. On the following day, compounds to be tested were solubilized in 100% DMSO to 100×the desired final testing concentration. Cells were incubated with compounds for 7 days at 37° C. in a 5% CO2 atmosphere before measurement of the luciferase readout. Cell viability (CC50) was measured with a CellTiter-Glo cell proliferation assay (Promega). |
| Affinity data for this assay | |
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