Assay Method Information | |
| ChEMBL_1294409 (CHEMBL3129194) |
Description: | Binding affinity to N-terminal GST-His-tagged recombinant human PAK1 kinase domain expressed in Escherichia coli BL21 using KKRNRRLSVA as sustrate preincubated for 10 mins followed by ATP addition measured after 60 mins by FRET-based Z'Lyte assay |
Affinity data for this assay | |
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