| Assay Method Information | |
| | ChEMBL_1644836 (CHEMBL3993765) |
| Description: | Non-competitive inhibition of recombinant human C-terminal His-tagged PHGDH (1 to 533 residues) expressed in Escherichia coli assessed as reduction in NADH formation preincubated for 30 mins followed by varying levels of 3-phosphoglycerate substrate addition in presence of constant NAD+ concentration by resorufin fluorescence based PSAT1/diaphorase/PSPH coupled enzyme assay |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |