Planarity and constraint of the carbonyl groups in 1,2-diones are determinants for selective inhibition of human carboxylesterase 1

J Med Chem. 2007 Nov 15;50(23):5727-34. doi: 10.1021/jm0706867. Epub 2007 Oct 17.

Abstract

Carboxylesterases (CE) are ubiquitous enzymes responsible for the detoxification of xenobiotics, including numerous clinically used drugs. Therefore, the selective inhibition of these proteins may prove useful in modulating drug half-life and bioavailability. Recently, we identified 1,2-diones as potent inhibitors of CEs, although little selectivity was observed in the inhibition of either human liver CE (hCE1) or human intestinal CE (hiCE). In this paper, we have further examined the inhibitory properties of ethane-1,2-diones toward these proteins and determined that, when the carbonyl oxygen atoms are cis-coplanar, the compounds demonstrate specificity for hCE1. Conversely, when the dione oxygen atoms are not planar (or are trans-coplanar), the compounds are more potent at hiCE inhibition. These properties have been validated in over 40 1,2-diones that demonstrate inhibitory activity toward at least one of these enzymes. Statistical analysis of the results confirms the correlation (P < 0.001) between the dione dihedral angle and the preferential inhibition of either hiCE or hCE1. Overall, the results presented here define the parameters necessary for small molecule inhibition of human CEs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholinesterase / chemistry
  • Butyrylcholinesterase / chemistry
  • Carboxylic Ester Hydrolases / antagonists & inhibitors*
  • Carboxylic Ester Hydrolases / chemistry
  • Crystallography, X-Ray
  • Glyoxal / analogs & derivatives*
  • Glyoxal / chemical synthesis*
  • Glyoxal / chemistry
  • Humans
  • Intestines / enzymology
  • Models, Molecular
  • Molecular Structure
  • Quantitative Structure-Activity Relationship

Substances

  • Glyoxal
  • Carboxylic Ester Hydrolases
  • CES1 protein, human
  • Acetylcholinesterase
  • Butyrylcholinesterase