Cloning and functional expression of the guinea pig neuropeptide Y Y2 receptor

Regul Pept. 1998 Sep 25:75-76:23-8. doi: 10.1016/s0167-0115(98)00049-4.

Abstract

Five neuropeptide Y (NPY) receptor subtypes have been cloned in mammals. The degree of sequence conservation differs considerably between subtypes as well as between evolutionary lineages. To shed further light on this, we have cloned the five NPY receptors in the guinea pig. Here, we report the cloning of the guinea pig Y2 receptor. The Y2 receptor is generally highly conserved, with 90-95% identity between different orders of mammals, including the guinea pig. The guinea pig receptor has a divergent cytoplasmic tail, indicating possible differences in regulation of signalling and/or down regulation. COS-7 cells transiently transfected with the gpY2 receptor show saturable 125I-PYY binding with a Kd = 6 pM. In displacement experiments, the gpY2 receptor was similar to the human and rat receptors with the following rank order of potencies: pNPY > pPYY > pNPY13-36 = pNPY22-36 >> [Leu31Pro34]NPY > BIBP3226. Thus, the guinea pig Y2 receptor is well conserved in comparison with human and rat with regard to both amino acid sequence and pharmacological profile.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arginine / analogs & derivatives
  • Arginine / metabolism
  • COS Cells
  • Cloning, Molecular
  • Conserved Sequence
  • Gene Expression
  • Guinea Pigs
  • Humans
  • In Vitro Techniques
  • Kinetics
  • Molecular Sequence Data
  • Neuropeptide Y / analogs & derivatives
  • Neuropeptide Y / metabolism
  • Peptide Fragments / metabolism
  • Peptide YY / metabolism
  • Rats
  • Receptors, Neuropeptide Y / chemistry
  • Receptors, Neuropeptide Y / genetics*
  • Receptors, Neuropeptide Y / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Transfection

Substances

  • BIBP 3226
  • Neuropeptide Y
  • Peptide Fragments
  • Receptors, Neuropeptide Y
  • Recombinant Proteins
  • neuropeptide Y2 receptor
  • Peptide YY
  • Arginine

Associated data

  • GENBANK/AF072821