ORG-31540 BDBM50604260 SR-90107A IC-851589 Fondaparin sodium Fondaparinux sodium Fondaparinux sodium identification ORG 31540 Quixidar FONDAPARINUX SODIUM SR 90107A Fondaparinux sodium for assay Arixtra
CHEBI:61033 Fondaparinux BDBM50511579
- Zhang, GQ; Jin, H; Zhao, Y; Guo, L; Gao, X; Wang, X; Tie, S; Shen, J; Wang, PG; Gan, H; Cui, H; Zhao, W An efficient anticoagulant candidate: Characterization, synthesis and in vivo study of a fondaparinux analogue Rrt1.17. Eur J Med Chem 126: 1039-1055 (2017)
- ChEMBL_2289897 Inhibition of recombinant human HPSE expressed in insect cells by fondaparinux assay
- ChEMBL_2296896 Inhibition of heparanase (unknown origin) incubated for 18 hrs by fondaparinux assay
- ChEMBL_2296903 Inhibition of human heparanase using fondaparinux as substrate incubated for 18 hrs by Polymer-H fluorescence assay
- ChEMBL_2285317 Inhibition of HPSE (unknown origin) using fondaparinux as substrate incubated for 18 hrs by fluorescence plate reader analysis
- ChEMBL_829715 (CHEMBL2061155) Inhibition of human recombinant heparanase after 2 to 24 hrs by WST1 dye based fondaparinux assay
- ChEMBL_1793531 (CHEMBL4265450) Inhibition of recombinant HPSE (unknown origin) using fondaparinux as substrate incubated for 3 hrs in absence of light by WST1 assay
- ChEMBL_1826461 (CHEMBL4326335) Inhibition of recombinant HPSE GS3 (unknown origin) using fondaparinux as substrate incubated for 3 hrs in absence of light by WST1 based colorimetry
- Heparanase Inhibition Assay Recombinant human active heparanase derived from Chinese hamster ovary cells was from R&D Systems. Bovine serum albumin-coated 96 well microplates (96F Maxisorp NNC #456537, Thermo Scientific) were used for the assays and were prepared by incubation of the plates with 1% (w/v) BSA dissolved in phosphate-buffered saline containing 0.05% (v/v) Tween-20 (PBST) at 37° C. for 1 h. The plates were washed three times with PBST, shaken dry, and stored for up to two weeks at 4° C. before use. Assay mixtures typically contained 42.5 mM sodium acetate buffer (pH 5.0), 0.8 nM heparanase, 100 μM fondaparinux (Arixtra , Aspen Pharmacare), 5% (v/v) DMSO, and varying concentrations of inhibitor in a total volume of 100 μL. Following initiation of the reaction by addition of fondaparinux, the plate was sealed with adhesive film and incubated at 37° C. for 20-24 h. 100 μL of 1.69 mM WST-1 (Dojindo) solution in 0.1 M NaOH was added to the assay mixture. The plate was resealed and developed by incubation at 60° C. for 1 h, and the absorbance was measured at 584 nm. For the enzymatic assays, test compounds were dissolved and added to the assay at varying concentrations to calculate the level of inhibition.