Query String: HYDROCORTISONE
BDBM50371265 HYDROCORTISONE CYPIONATE Cortef Hydrocortisone Cyclopentylpropionate
CHEBI:31677 BDBM50016931 HYDROCORTISONE HEMISUCCINATE NSC-7576 Hydrocortisone Succinate
Hemsol-Hc BDBM50474607 Micort-Hc Hydrocortisone 21-acetate Neo-Cortef CHEBI:17609 NSC-741 Gppe Eye Crm Dricort Cortucid Hepacort Plus Orabase Hca Epifoam Genticin HC Barquinol HC Gentisone HC Framycort Hc45 Hydrocort Hydrocortone Gppe Foam Aero Chloromycetin Hydrocort Lanacort Hydrocortisone Acetate Cortifoam Gppe Ear Susp Cortril Fucidin H Cortef Acetate Colifoam Hydrocal
cortisol BDBM13775 US10188667, Example 00023 [3H]cortisol (1S,2R,10S,11S,14R,15S,17S)-14,17-dihydroxy-14-(2-hydroxyacetyl)-2,15-dimethyltetracyclo[8.7.0.0^{2,7}.0^{11,15}]heptadec-6-en-5-one 3H-cortisol HYDROCORTISONE 11beta,17alpha,21-Trihydroxy-4-pregnene-3,20-dione
- ChEMBL_39354 (CHEMBL654960) Beta-adrenergic agonistic activity in rat proximal colon in presence of phentolamine (10 uM), desmethylimipramine (0.5 uM) and hydrocortisone (30 uM)
- HepaRG-CAR Cell-Based Assay for Quantitation of Glycolate Oxidase Inhibition A HepaRG human hepatic cell line was transfected for stable overexpression of the constitutive androstane receptor (i.e., HepaRG-CAR cells), as reported by van der Mark et al. (Drug Metab. Dispos., 2017, 45:56-67. Overexpression of CAR in these cells resulted in higher levels of glycolate oxidase (GOX) expression compared to the parental HepaRG cells. HepaRG-CAR cells were plated in a 12-wells plate and incubated for 4 weeks until fully differentiated.To measure cellular glycolate flux, the HepaRG-CAR cells were incubated in Williams medium supplemented with 10% fetal bovine serum (FBS), 5 μg/mL insulin, 50 μM hydrocortisone hemisuccinate, 2 mM glutamine, 5000 U/mL penicillin and 5 mg/mL streptomycin. Test compounds were added to the medium at 0, 0.3, 1, 3 or 10 μM and incubated for 30 minutes, after which 500 μM glycolate was also added. After incubation for 48 hours, 400 μL medium was taken from the culture plate and added to 60 μL 37% HCl.Internal standards (2,2-d2 glycolate, 1,2-13C2 oxalate and 13C2-glyoxylate) and hydroxylamine were added followed by another 30 minute incubation at 80° C. The acids were extracted using ethyl acetate with NaCl. The organic phase was dried under nitrogen and derivatized with N-tert-butyldimethylsilyl-N-methyl trifluoroacetamide (MTBSTFA) for 30 minutes at 80° C. The amounts of glycolate, glyoxylate and oxalate were determined by gas chromatography-mass spectrometry (GC-MS) analysis, using a 25 meter CP-Sil 5 CB low bleed column. A standard curve was used to calculate the concentrations of each acid in the culture medium.