[3H]-Prazosin BDBM29568 CHEMBL2 PRAZOSIN [3H]-Furazosin [3H]-Pratsiol [3H]-Minipress PRAZOSIN HYDROCHLORIDE
CHEMBL1558 [4-(4-Amino-6,7-dimethoxy-quinazolin-2-yl)-piperazin-1-yl]-furan-2-yl-methanone; hydrochloride Minipress xl Prazosin BDBM50299207 [4-(4-Amino-6,7-dimethoxy-quinazolin-2-yl)-piperazin-1-yl]-furan-2-yl-methanone; hydrochloride (Prazosin) Minipress CP-12299-1 PRAZOSIN HYDROCHLORIDE [4-(4-Amino-6,7-dimethoxy-quinazolin-2-yl)-piperazin-1-yl]-furan-2-yl-methanone; hydrochloride (Prazocin)
- Pitha, J; Szabo, L; Szurmai, Z; Buchowiecki, W; Kusiak, JW Alkylating prazosin analogue: irreversible label for alpha 1-adrenoceptors. J Med Chem 32: 96-100 (1989)
- Greengrass, P; Bremner, R Binding characteristics of 3H-prazosin to rat brain alpha-adrenergic receptors. Eur J Pharmacol 55: 323-6 (1979)
- Rosini, M; Antonello, A; Cavalli, A; Bolognesi, ML; Minarini, A; Marucci, G; Poggesi, E; Leonardi, A; Melchiorre, C Prazosin-related compounds. Effect of transforming the piperazinylquinazoline moiety into an aminomethyltetrahydroacridine system on the affinity for alpha1-adrenoreceptors. J Med Chem 46: 4895-903 (2003)
- Bolognesi, ML; Marucci, G; Angeli, P; Buccioni, M; Minarini, A; Rosini, M; Tumiatti, V; Melchiorre, C Analogues of prazosin that bear a benextramine-related polyamine backbone exhibit different antagonism toward alpha1-adrenoreceptor subtypes. J Med Chem 44: 362-71 (2001)
- Winters, G; Sala, A; Barone, D; Baldoli, E Synthesis, in vitro [3H]prazosin displacement, and in vivo activity of 3-aryl-4,5,6,7-tetrahydropyrazolo[4,3-c]pyridines, a new class of antihypertensive agents. J Med Chem 28: 934-40 (1985)
- Jones, SB; Smith, JM; Jones, AW; Bylund, DB Alpha-1 adrenergic receptor binding in aortas from rat and dog: comparison of [3H]prazosin and beta-iodo-[125I]-4-hydroxyphenyl-ethyl-aminomethyl-tetralone. J Pharmacol Exp Ther 241: 875-81 (1987)
- ChEBML_1696080 Displacement of [3H]prazosin from human sigma1 receptor
- ChEMBL_1552598 (CHEMBL3762403) Displacement of [3H]-prazosin from human ADRA1A receptor
- ChEMBL_1661757 (CHEMBL4011369) Displacement of [3H]Prazosin from human adrenergic alpha1A receptor
- ChEMBL_1661758 (CHEMBL4011370) Displacement of [3H]Prazosin from human adrenergic alpha1B receptor
- ChEMBL_1661759 (CHEMBL4011371) Displacement of [3H]Prazosin from human adrenergic alpha1D receptor
- ChEMBL_303405 (CHEMBL839162) Inhibition of [3H]- prazosin binding to alpha-1 adrenergic receptor
- ChEMBL_33239 (CHEMBL645798) Displacement of [3H]prazosin from alpha-1 adrenergic receptor
- ChEMBL_33424 (CHEMBL648820) Displacement of [3H]prazosin from Alpha-1 adrenergic receptor
- ChEMBL_439676 (CHEMBL888787) Displacement of [3H]prazosin from adrenergic alpha-1A receptor
- ChEMBL_439677 (CHEMBL888788) Displacement of [3H]prazosin from adrenergic alpha-1B receptor
- ChEMBL_594566 (CHEMBL1037061) Displacement of [3H]prazosin from human adrenergic alpha1A receptor
- ChEMBL_604277 (CHEMBL1066733) Displacement of [3H]Prazosin from human adrenergic alpha1A receptor
- ChEMBL_652614 (CHEMBL1225817) Displacement of [3H]prazosin from human adrenergic alpha1A receptor
- ChEBML_1696907 Displacement of [3H]prazosin from rat salivary gland adrenergic alpha1a receptor
- ChEBML_32441 Displacement of [3H]- prazosin from human recombinant Alpha-1D adrenergic receptor
- ChEBML_33727 Displacement of [3H]- prazosin from human recombinant Alpha-1A adrenergic receptor
- ChEBML_34341 Displacement of [3H]- prazosin from human recombinant Alpha-1B adrenergic receptor
- ChEMBL_1513484 (CHEMBL3611840) Displacement of [3H]prazosin from alpha1B adrenoceptor in rat liver membranes
- ChEMBL_1823723 (CHEMBL4323487) Displacement of [3H] prazosin from alpha1A adrenergic receptor (unknown origin)
- ChEMBL_2023659 (CHEMBL4677472) Displacement of [3H]-prazosin from adrenergic alpha1 in rat cerebral cortex
- ChEMBL_32713 (CHEMBL644031) Binding affinity against isolated human aorta using [3H]- prazosin.
- ChEMBL_33430 (CHEMBL648826) Ability to displace [3H]-Prazosin binding to Alpha-1 adrenergic receptor
- ChEMBL_33891 (CHEMBL645469) Binding affinity against isolated human prostate using [3H]- prazosin.
- ChEMBL_34177 (CHEMBL648416) Binding affinity against isolated rat prostate using [3H]- prazosin.
- ChEMBL_34573 (CHEMBL649337) Binding affinity to Alpha-1 adrenergic receptor by displacement of [3H]prazosin
- ChEMBL_436130 (CHEMBL905532) Displacement of [3H]prazosin from human cloned alpha-1A receptor
- ChEMBL_502633 (CHEMBL989308) Displacement of [3H]prazosin from alpha1 adrenoceptor in rat heart membrane
- ChEMBL_597655 (CHEMBL1039954) Displacement of [3H]prazosin from rat salivary gland alpha1A adrenoceptor
- ChEMBL_604278 (CHEMBL1066734) Displacement of [3H]Prazosin from human adrenergic Alpha-1B receptor
- ChEBML_1161 Displacement of [3H]prazosin from rat cortex membrane 5-hydroxytryptamine 1A receptor
- ChEBML_33096 Displacement of [3H]prazosin from calf cerebral cortex alpha-1 adrenergic receptor
- ChEBML_33452 Binding affinity for Alpha-1 adrenergic receptor was determined by using [3H]prazosin as radioligand
- ChEMBL_1365250 (CHEMBL3293215) Displacement of [3H]prazosin from human adrenergic-alpha1A receptor by PDSP assay
- ChEMBL_1513483 (CHEMBL3611839) Displacement of [3H]prazosin from alpha1A adrenoceptor in rat submaxillary gland membrane
- ChEMBL_1744659 (CHEMBL4179169) Displacement of [3H]prazosin from adrenergic alpha1 receptor in rat cerebral cortex
- ChEMBL_2058189 (CHEMBL4713190) Displacement of [3H]prazosin from alpha-1A adrenergic receptor (unknown origin)
- ChEMBL_2197420 (CHEMBL5109936) Displacement of [3H]prazosin from rat cerebral cortex alpha1 adrenergic receptor
- ChEMBL_2197465 (CHEMBL5109981) Displacement of [3H]prazosin from rat whole brain alpha1 adrenergic receptor
- ChEMBL_32441 (CHEMBL646102) Displacement of [3H]- prazosin from human recombinant Alpha-1D adrenergic receptor
- ChEMBL_32501 (CHEMBL641378) Inhibition of binding of [3H]-prazosin at alpha1 adrenoceptors isolated from rat brain
- ChEMBL_32588 (CHEMBL643425) Displacement of [3H]prazosin from human cloned Alpha-1D adrenergic receptor
- ChEMBL_326680 (CHEMBL868696) Displacement of [3H]prazosin from cloned human ADRA1A expressed in CHO cells
- ChEMBL_326681 (CHEMBL868697) Displacement of [3H]prazosin from cloned human ADRA1B expressed in CHO cells
- ChEMBL_326682 (CHEMBL868698) Displacement of [3H]prazosin from cloned human ADRA1D expressed in CHO cells
- ChEMBL_33595 (CHEMBL652804) In vitro for the displacement of [3H]prazosin binding to bovine Alpha-1A adrenergic receptor
- ChEMBL_33727 (CHEMBL647043) Displacement of [3H]- prazosin from human recombinant Alpha-1A adrenergic receptor
- ChEMBL_33874 (CHEMBL643746) Displacement of [3H]prazosin from human cloned Alpha-1A adrenergic receptor
- ChEMBL_340158 (CHEMBL865593) Displacement of [3H]prazosin from alpha-1 adrenergic receptor in rat cortex
- ChEMBL_34341 (CHEMBL649154) Displacement of [3H]- prazosin from human recombinant Alpha-1B adrenergic receptor
- ChEMBL_34485 (CHEMBL651052) Displacement of [3H]prazosin from human cloned Alpha-1B adrenergic receptor
- ChEMBL_446518 (CHEMBL895630) Displacement of [3H]prazosin from adrenergic alpha1 receptor in rat cerebral cortex
- ChEMBL_574168 (CHEMBL1060369) Displacement of [3H]prazosin from human alpha1A adrenoceptor expressed in CHO cells
- ChEMBL_663467 (CHEMBL1251066) Displacement of [3H]prazosin from human adrenergic alpha1A expressed in CHO cells
- ChEMBL_693737 (CHEMBL1638256) Displacement of [3H]prazosin from human alpha1A adrenoceptor expressed in CHO cells
- ChEMBL_911270 (CHEMBL3058318) Displacement of [3H]prazosin from Homo sapiens (human) alpha1D adrenergic receptor
- ChEMBL_911271 (CHEMBL3058319) Displacement of [3H]prazosin from Homo sapiens (human) alpha1B adrenergic receptor
- ChEMBL_911272 (CHEMBL3058320) Displacement of [3H]prazosin from Homo sapiens (human) alpha1A adrenergic receptor
- ChEMBL_989362 (CHEMBL2443397) Displacement of [3H]-prazosin from alpha1-adrenergic receptor in pig cerebral cortex
- ChEBML_33271 Displacement of [3H]prazosin from Alpha-1 adrenergic receptor in whole rat brain membranes
- ChEBML_33282 The ability to inhibit [3H]prazosin binding to Alpha-1 adrenergic receptor in rat whole brain
- ChEBML_33438 In vitro binding affinity towards Alpha-1 adrenergic receptor by displacing [3H]prazosin radioligand
- ChEMBL_1366829 (CHEMBL3297339) Displacement of [3H]prazosin from human recombinant alpha1A adrenoceptor expressed in CHO cells
- ChEMBL_1436282 (CHEMBL3387782) Displacement of [3H]-prazosin from human alpha1A adrenergic receptor transfected in COS1 cells
- ChEMBL_1436284 (CHEMBL3387784) Displacement of [3H]-prazosin from human alpha1B adrenergic receptor transfected in COS1 cells
- ChEMBL_1436286 (CHEMBL3387786) Displacement of [3H]-prazosin from human alpha1D adrenergic receptor transfected in COS1 cells
- ChEMBL_1468615 (CHEMBL3413561) Displacement of [3H]prazosin from human adrenergic alpha1A receptor expressed in CHO cells
- ChEMBL_216961 (CHEMBL822779) Binding affinity towards alpha receptor in rat brain using [3H]prazosin as radioligand
- ChEMBL_218071 (CHEMBL822104) Inhibition of saturable binding of [3H]prazosin to alpha1-site in rat cerebral cortical membranes.
- ChEMBL_30088 (CHEMBL642867) Displacement of [3H]prazosin from alpha-1 adrenoceptors in whole rat brain membranes
- ChEMBL_30097 (CHEMBL873041) In vitro binding affinity using [3H]prazosin as radioligand against adrenoceptor alpha 1A
- ChEMBL_306566 (CHEMBL829141) Concentration required for inhibition of alpha-1 adrenergic receptor using [3H]prazosin as the radioligand
- ChEMBL_32313 (CHEMBL646167) Binding affinity towards human Alpha-1C adrenergic receptor by the displacement of [3H]prazosin
- ChEMBL_32428 (CHEMBL646090) Ability to displace [3H]prazosin from cloned human Alpha-1D adrenergic receptor
- ChEMBL_32548 (CHEMBL645683) In vitro binding affinity determined for alpha-1 adrenergic receptor by the displacement of [3H]- prazosin.
- ChEMBL_32554 (CHEMBL645689) Displacement of [3H]prazosin from Alpha-1 adrenergic receptor of rat cerebral cortex
- ChEMBL_32601 (CHEMBL642128) Binding affinity determined by displacement of [3H]prazosin from alpha-1D adrenergic receptor
- ChEMBL_33014 (CHEMBL643870) Ability to displace [3H]prazosin from human cloned Alpha-1B adrenergic receptor
- ChEMBL_33023 (CHEMBL643948) Ability to displace [3H]prazosin from human cloned Alpha-1D adrenergic receptor
- ChEMBL_33102 (CHEMBL645955) Inhibition of [3H]prazosin binding to Alpha-1 adrenergic receptor in bovine frontal cortex
- ChEMBL_33159 (CHEMBL642944) Compound was tested for i the displacement of [3H]prazosin from alpha-1 adrenergic receptor
- ChEMBL_33418 (CHEMBL648814) The compound was evaluated for the ability to displace [3H]prazosin binding to Alpha-1 adrenergic receptor
- ChEMBL_33603 (CHEMBL652811) Ability to displace [3H]prazosin from cloned human Alpha-1A adrenergic receptor
- ChEMBL_33857 (CHEMBL646435) Displacement of [3H]prazosin from Alpha-1 adrenergic receptor in rat frontal cortex
- ChEMBL_33881 (CHEMBL643753) Binding affinity towards human Alpha-1A adrenergic receptor by the displacement of [3H]prazosin
- ChEMBL_33886 (CHEMBL643758) Binding affinity determined by displacement of [3H]prazosin from alpha-1A adrenergic receptor
- ChEMBL_34005 (CHEMBL643634) Inhibition of binding of [3H]prazosin to Alpha-1 adrenergic receptor in rat cortical membranes
- ChEMBL_34326 (CHEMBL648109) Ability to displace [3H]prazosin from cloned human Alpha-1B adrenergic receptor
- ChEMBL_34340 (CHEMBL649153) Displacement of [3H]prazosin from Alpha-1B adrenergic receptor of rat liver membrane
- ChEMBL_34423 (CHEMBL649427) Displacement of [3H]-prazosin from Alpha-1 adrenergic receptor of rat brain membranes
- ChEMBL_34433 (CHEMBL649436) Binding affinity for alpha-1 adrenergic receptor in rat liver using [3H]prazosin.
- ChEMBL_34449 (CHEMBL651981) Displacement of [3H]prazosin from alpha-1 adrenergic receptor of rat brain homogenate
- ChEMBL_34597 (CHEMBL645556) Displacement of [3H]prazosin from Alpha-1 adrenergic receptor of rat cortex membranes
- ChEMBL_34607 (CHEMBL645566) Binding affinity towards human Alpha-1B adrenergic receptor by the displacement of [3H]prazosin
- ChEMBL_34615 (CHEMBL645573) Binding affinity determined by displacement of [3H]prazosin from alpha-1B adrenergic receptor
- ChEMBL_384613 (CHEMBL866127) Displacement of [3H]prazosin from adrenergic alpha-1B receptor in rat liver membranes
- ChEMBL_461983 (CHEMBL944826) Displacement of [3H]prazosin from rat adrenergic alpha1A receptor expressed in fibroblast cells
- ChEMBL_516617 (CHEMBL991393) Displacement of [3H]prazosin from adrenergic Alpha-1B receptor expressed in CHO cells
- ChEMBL_539780 (CHEMBL1034998) Displacement of [3H]prazosin from human cloned alpha1A adrenoceptor expressed in CHO cells
- ChEMBL_539782 (CHEMBL1035000) Displacement of [3H]prazosin from human cloned alpha1D adrenoceptor expressed in CHO cells
- ChEMBL_558279 (CHEMBL956579) Displacement of [3H]prazosin from human adrenergic alpha1A receptor expressed in LMtk- cells
- ChEMBL_574169 (CHEMBL1060370) Displacement of [3H]prazosin from human Alpha-1B adrenoceptor expressed in CHO cells
- ChEMBL_574170 (CHEMBL1060371) Displacement of [3H]prazosin from human Alpha-1D adrenoceptor expressed in CHO cells
- ChEMBL_615772 (CHEMBL1100126) Displacement of [3H]prazosin from human alpha1A adrenergic receptor expressed in CHO cells
- ChEMBL_663468 (CHEMBL1251067) Displacement of [3H]prazosin from human adrenergic Alpha-1B expressed in CHO cells
- ChEMBL_663469 (CHEMBL1251068) Displacement of [3H]prazosin from human adrenergic Alpha-1D expressed in CHO cells
- ChEMBL_693738 (CHEMBL1638257) Displacement of [3H]prazosin from human Alpha-1B adrenoceptor expressed in CHO cells
- ChEMBL_693739 (CHEMBL1638258) Displacement of [3H]prazosin from human Alpha-1D adrenoceptor expressed in CHO cells
- ChEMBL_791620 (CHEMBL1930844) Displacement of [3H]prazosin from pig adrenergic alpha1 receptor in cerebral cortex homogenate
- ChEBML_1711419 Displacement of [3H]-prazosin from rat alpha1 adrenoceptor after 60 mins by scintillation counting analysis
- ChEBML_33091 Affinity to alpha-1 adrenergic receptor by the displacement of [3H]-prazosin from calf cerebral cortex membranes
- ChEBML_33098 Displacement of [3H]- prazosin radioligand binding at the alpha 1-adrenergic binding site of calf cerebral cortex
- ChEBML_33099 Evaluated for its ability to displace [3H]prazosin from alpha-1-Adrenoceptor of calf cerebral cortex
- ChEBML_33274 Compound was tested for the displacement of [3H]prazosin from alpha-1 adrenergic receptor of rat striatal membranes
- ChEMBL_1290914 (CHEMBL3118887) Displacement of [3H]-prazosin from rat brain alpha1 adrenergic receptor after 50 mins
- ChEMBL_1436373 (CHEMBL3389576) Inhibition of BCRP (unknown origin) expressed in T8 cells using Bodipy FL-prazosin substrate
- ChEMBL_164466 (CHEMBL770732) Inhibition of specific [3H]-prazosin binding (0.2 nM) to rat brain membranes alpha1 adrenoceptor.
- ChEMBL_164995 (CHEMBL770198) Inhibition of specific [3H]prazosin binding (0.2 nM) to rat brain membranes alpha1 adrenoceptor.
- ChEMBL_216130 (CHEMBL816950) Binding affinity towards human cloned alpha-1A-adrenoceptor using [3H]prazosin as radioligand
- ChEMBL_216132 (CHEMBL816952) Binding affinity towards human cloned alpha-1B-adrenoceptor using [3H]prazosin as radioligand
- ChEMBL_216134 (CHEMBL816954) Binding affinity towards human cloned alpha-1D-adrenoceptor using [3H]prazosin as radioligand
- ChEMBL_216442 (CHEMBL820157) In vitro binding affinity towards alpha-adreno ceptor of rat forebrain using [3H]prazosin
- ChEMBL_216443 (CHEMBL820158) In vitro binding affinity towards alpha-adreno ceptor of rat forebrain using [3H]prazosin
- ChEMBL_218069 (CHEMBL821941) Binding affinity against alpha-1-adrenoceptor in rat brain homogenates [3H]-prazosin displacement.
- ChEMBL_2199780 (CHEMBL5112296) Displacement of [3H]-prazosin from human adrenergic alpha1A receptor expressed in human HEK293 cells
- ChEMBL_32404 (CHEMBL648040) Ability to displace [3H]prazosin from postsynaptic alpha-1 adrenergic receptor of rat in vitro.
- ChEMBL_32416 (CHEMBL876575) In vitro binding affinity towards Alpha-1 adrenergic receptor was determined by the displacement of [3H]prazosin
- ChEMBL_32443 (CHEMBL646104) Inhibition of [3H]prazosin binding to human Alpha-1D adrenergic receptor expressed in CHO cells
- ChEMBL_32559 (CHEMBL645694) Binding affinity against alpha-1 adrenergic receptor in rat using [3H]prazosin as radioligand
- ChEMBL_32561 (CHEMBL645695) Binding affinity against Alpha-1 adrenergic receptor in rat cortex using [3H]prazosin
- ChEMBL_33071 (CHEMBL647970) Compound is evaluated for binding affinity towards Alpha-2A adrenergic receptor using [3H]prazosin
- ChEMBL_33136 (CHEMBL642091) Binding affinity at Alpha-1 adrenergic receptor by inhibition of [3H]-prazosin binding to rat cortex
- ChEMBL_33140 (CHEMBL642095) Binding affinity towards Alpha-1 adrenergic receptor using [3H]prazosin in rat heart
- ChEMBL_33141 (CHEMBL642096) Binding affinity towards Alpha-1 adrenergic receptor using prazosin as a radioligand in rat cortex
- ChEMBL_33234 (CHEMBL645793) In vitro binding affinity against Alpha-1 adrenergic receptor using [3H]prazosin as radioligand
- ChEMBL_33272 (CHEMBL643610) Displacement of [3H]prazosin from Alpha-1 adrenergic receptor of whole rat brain membranes
- ChEMBL_33275 (CHEMBL643613) Binding affinity for rat cortex alpha-1 adrenergic receptor by inhibition of [3H]prazosin binding
- ChEMBL_33282 (CHEMBL644242) The ability to inhibit [3H]prazosin binding to Alpha-1 adrenergic receptor in rat whole brain
- ChEMBL_33353 (CHEMBL646283) Compound is evaluated for binding affinity towards Alpha-2B adrenergic receptor using [3H]prazosin
- ChEMBL_33429 (CHEMBL648825) In vitro binding affinity towards Alpha-1 adrenergic receptor by displacing [3H]prazosin radioligand
- ChEMBL_33455 (CHEMBL649180) Compound was tested for binding affinity using [3H]prazosin against Alpha-1 adrenergic receptor
- ChEMBL_33591 (CHEMBL858019) The compound was screened in vitro for the displacement of [3H]prazosin binding to bovine Alpha-1A adrenergic receptor
- ChEMBL_33726 (CHEMBL647547) Displacement of [3H]prazosin from Alpha-1A adrenergic receptor of rat submaxillary gland membranes
- ChEMBL_33729 (CHEMBL647045) Inhibition of [3H]prazosin binding to human Alpha-1A adrenergic receptor expressed in CHO cells
- ChEMBL_33999 (CHEMBL643628) Inhibition of [3H]prazosin binding to alpha-1 adrenergic receptor from rat cortical membranes
- ChEMBL_34002 (CHEMBL643631) Inhibition of specific [3H]prazosin binding (0.2 nM) to rat brain membranes alpha1 adrenoceptor.
- ChEMBL_34033 (CHEMBL646286) In vitro binding affinity using [3H]prazosin as radioligand against alpha-1A adrenergic receptor
- ChEMBL_34135 (CHEMBL644129) Tested for the Alpha-1 adrenergic receptor affinity in a standard radioligand binding assay with [3H]- prazosin
- ChEMBL_34180 (CHEMBL648419) Inhibitory constant against alpha-1A receptor using [3H]-7-MeO prazosin radioligand
- ChEMBL_34343 (CHEMBL649156) Inhibition of [3H]prazosin binding to human Alpha-1B adrenergic receptor expressed in CHO cells
- ChEMBL_34441 (CHEMBL647021) Binding affinity at Alpha-1 adrenergic receptors on rat cortical membranes by [3H]prazosin displacement.
- ChEMBL_34446 (CHEMBL651978) Binding affinity towards Alpha-1 adrenergic receptor was measured through displacement of [3H]prazosin
- ChEMBL_34572 (CHEMBL649336) Binding affinity at Alpha-1 adrenergic receptors on rat cortical membranes by [3H]prazosin displacement.
- ChEMBL_34574 (CHEMBL872367) Binding affinity towards Alpha-1 adrenergic receptor in rat brain using [3H]prazosin
- ChEMBL_34635 (CHEMBL647280) Inhibition of [3H]- prazosin binding against Alpha-1B adrenergic receptor from rat liver
- ChEMBL_34642 (CHEMBL647287) Inhibition of [3H]- prazosin binding against Alpha-1B adrenergic receptor from rat liver
- ChEMBL_357739 (CHEMBL870198) Displacement of [3H]prazosin from adrenergic alpha-1 receptor in rat cerebral cortex cells
- ChEMBL_384612 (CHEMBL866126) Displacement of [3H]prazosin from adrenergic alpha-1A receptor in rat submaxillary gland membranes
- ChEMBL_504110 (CHEMBL982305) Displacement of [3H]prazosin from alpha1 adrenergic receptor in Wistar rat cerebral cortex membrane
- ChEMBL_539781 (CHEMBL1034999) Displacement of [3H]prazosin from human cloned Alpha-1B adrenoceptor expressed in CHO cells
- ChEMBL_603940 (CHEMBL1043293) Displacement of [3H]prazosin from human adrenergic beta2 receptor expressed in CHO cell membrane
- ChEMBL_615773 (CHEMBL1100127) Displacement of [3H]prazosin from human Alpha-1B adrenergic receptor expressed in CHO cells
- ChEMBL_615925 (CHEMBL1099771) Displacement of [3H]prazosin from human Alpha-1D adrenergic receptor expressed in CHO cells
- ChEBML_33142 Binding affinity determined to alpha-1 adrenergic receptor of rat cerebral cortex using [3H]prazosin as radioligand
- ChEMBL_218068 (CHEMBL821940) Binding affinity against alpha-1-adrenoceptor was determined by displacement of [3H]-prazosin in rat brain homogenates.
- ChEMBL_2197427 (CHEMBL5109943) Displacement of [3H]-prazosin from rat whole cortex membrane homogenate alpha 1 adrenergic receptor
- ChEMBL_2324649 Displacement of [3H]-Prazosin from human adrenergic alpha1B receptor assessed as inhibition constant by radioligand displacement assay
- ChEMBL_303469 (CHEMBL838844) In vitro inhibition of [3H]prazosin binding to Alpha-1 adrenergic receptor in rat cerebral cortex membranes
- ChEMBL_32295 (CHEMBL646242) Compound was tested for binding affinity against Alpha-1B adrenergic receptor (rat liver, [3H] prazosin)
- ChEMBL_32413 (CHEMBL648048) In vitro binding affinity at Alpha-1 adrenergic receptor by radioligand binding assay using [3H]prazosin
- ChEMBL_32430 (CHEMBL646092) Binding affinity against Alpha-1D adrenergic receptor (recombinant human receptor) using [3H]-prazosin.
- ChEMBL_32542 (CHEMBL646576) Inhibition of saturable binding of [3H]prazosin to alpha-1 adrenergic receptor in rat cerebral cortical membranes.
- ChEMBL_32558 (CHEMBL645693) The compound was evaluated for the inhibition of [3H]prazosin binding to alpha-1 adrenergic receptor from rat liver
- ChEMBL_32723 (CHEMBL645995) Binding affinity towards Alpha-1D adrenergic receptor in rat brain using [3H]prazosin as radioligand
- ChEMBL_33091 (CHEMBL643583) Affinity to alpha-1 adrenergic receptor by the displacement of [3H]-prazosin from calf cerebral cortex membranes
- ChEMBL_33104 (CHEMBL645957) Binding affinity towards Alpha-1 adrenergic receptor of calf brain cortex using [3H]prazosin
- ChEMBL_33146 (CHEMBL642101) In vitro binding affinity against alpha-1 adrenergic receptor of rat in presence of [3H]-prazosin radioligand
- ChEMBL_33301 (CHEMBL646154) Affinity for alpha-1 adrenoceptor of rat cerebral cortex was determined by ligand binding using [3H]prazosin
- ChEMBL_33445 (CHEMBL649610) Compound was tested for the binding affinity against Alpha-1 adrenergic receptor by using [3H]prazosin as radioligand
- ChEMBL_33561 (CHEMBL648423) Displacement of [3H]prazosin from alpha-1 adrenergic receptor from rat brain cerebral cortex
- ChEMBL_33585 (CHEMBL647136) Binding affinity towards Alpha-1A adrenergic receptor in bovine brain using [3H]-prazosin as radioligand
- ChEMBL_33586 (CHEMBL647137) Binding affinity towards Alpha-1A adrenergic receptor in bovine brain using [3H]prazosin as radioligand
- ChEMBL_33605 (CHEMBL876765) Binding affinity against Alpha-1A adrenergic receptor (recombinant human receptor) using [3H]prazosin.
- ChEMBL_33691 (CHEMBL647162) Affinity for alpha-1 adrenoceptor of rat cerebral cortex was determined by ligand binding using [3H]prazosin.
- ChEMBL_34016 (CHEMBL645383) Inhibition of [3H]- prazosin binding against Alpha-1A adrenergic receptor from rat submaxillary gland
- ChEMBL_34034 (CHEMBL646287) Inhibition of [3H]- prazosin binding against Alpha-1A adrenergic receptor from rat submaxillary gland
- ChEMBL_34140 (CHEMBL644779) The compound was evaluated for the inhibition of [3H]prazosin binding to alpha-1 adrenergic receptor from rat liver
- ChEMBL_34178 (CHEMBL648417) Inhibitory constant against Alpha-1A adrenergic receptor using [3H]-7-MeO prazosin radioligand
- ChEMBL_34179 (CHEMBL648418) Inhibitory constant against Alpha-1B adrenergic receptor using [3H]-7-MeO prazosin radioligand
- ChEMBL_34299 (CHEMBL652743) Ability to displace [3H]prazosin from alpha-1 adrenergic receptor in rat brain homogenates in vitro
- ChEMBL_34328 (CHEMBL648111) Binding affinity against Alpha-1B adrenergic receptor (recombinant human receptor) using [3H]prazosin.
- ChEMBL_34577 (CHEMBL882498) Binding affinity towards Alpha-1 adrenergic receptor in rat cerebral cortex using [3H]prazosin
- ChEMBL_34598 (CHEMBL645557) Compound was evaluated for inhibition of [3H]prazosin binding to Alpha-1 adrenergic receptor in rat forebrain homogenate.
- ChEMBL_34599 (CHEMBL645558) Alpha-1 adrenergic receptor binding affinity by measuring the displacement of [3H]prazosin binding in rat brain
- ChEMBL_34601 (CHEMBL645560) Binding affinity at Alpha-1 adrenergic receptor in rat cerebral cortex membranes by [3H]prazosin displacement.
- ChEMBL_34636 (CHEMBL647281) Binding affinity towards Alpha-1B adrenergic receptor in rat liver using [3H]prazosin as radioligand
- ChEMBL_412940 (CHEMBL910638) Displacement of [3H]prazosin from cloned human adrenergic alpha 1A receptor expressed in CHO cells
- ChEMBL_412941 (CHEMBL910640) Displacement of [3H]prazosin from cloned human adrenergic alpha 1B receptor expressed in CHO cells
- ChEMBL_412942 (CHEMBL910642) Displacement of [3H]prazosin from cloned human adrenergic alpha 1D receptor expressed in CHO cells
- ChEMBL_603939 (CHEMBL1043292) Displacement of [3H]prazosin from human adrenergic Alpha-1B receptor expressed in CHO cell membrane
- ChEMBL_774872 (CHEMBL1913214) Displacement of [3H]prazosin from alpha1A receptor expressed in HEK293 EBNA cells by radioligand binding assay
- ChEMBL_935398 (CHEMBL2318947) Displacement of [3H]-prazosin from alpha1-adrenergic receptor in rat cerebral cortex by liquid scintillation counting
- ChEBML_33034 Binding affinity against alpha-2 adrenergic receptor from calf cerebral cortex, using [3H]prazosin as the radioligand
- ChEBML_33092 Binding activity against alpha-1 adrenergic receptor from calf cerebral cortex, using [3H]-prazosin as the radioligand
- ChEBML_33866 Binding affinity at human Alpha-1A adrenergic receptor in CHO cells uby [3H]prazosin (0.25 nM) displacement.
- ChEMBL_164465 (CHEMBL770731) Inhibition of specific [3H]-prazosin binding (0.2 nM) to rat brain membranes alpha-1 adrenergic receptor
- ChEMBL_1723940 (CHEMBL4139218) Displacement of [3H]prazosin from human alpha1A adrenoceptor expressed in CHO cell membranes after 30 mins
- ChEMBL_1723941 (CHEMBL4139219) Displacement of [3H]prazosin from human alpha1B adrenoceptor expressed in CHO cell membranes after 30 mins
- ChEMBL_1723942 (CHEMBL4139220) Displacement of [3H]prazosin from human alpha1D adrenoceptor expressed in CHO cell membranes after 30 mins
- ChEMBL_1726507 (CHEMBL4141785) Displacement of [3H]-prazosin from rat alpha1A adrenergic receptor after 60 mins by scintillation counting analysis
- ChEMBL_1755196 (CHEMBL4189956) Displacement of [3H]-Prazosin from human adrenergic alpha1A receptor after 90 mins by scintillation counting method
- ChEMBL_1755197 (CHEMBL4189957) Displacement of [3H]-Prazosin from human adrenergic alpha1B receptor after 90 mins by scintillation counting method
- ChEMBL_1755198 (CHEMBL4189958) Displacement of [3H]-Prazosin from human adrenergic alpha1D receptor after 90 mins by scintillation counting method
- ChEMBL_1760110 (CHEMBL4195118) Displacement of [3H]prazosin from rat cerebral cortex adrenergic receptor alpha1 by liquid scintillation counting method
- ChEMBL_216005 (CHEMBL820507) Binding affinity towards alpha-1 adrenergic receptor using [3H]prazosin as radioligand in competitive binding assay
- ChEMBL_221952 (CHEMBL843517) Inhibition of saturable binding of [3H]prazosin to alpha-1 adrenergic receptor site in rat cerebral cortical membranes.
- ChEMBL_30103 (CHEMBL642030) In vitro binding affinity using [3H]prazosin as radioligand against adrenoceptor alpha 1b expressed in LTK cell
- ChEMBL_30104 (CHEMBL642031) In vitro binding affinity using [3H]prazosin as radioligand against adrenoceptor alpha 1d expressed in LTK cell
- ChEMBL_302885 (CHEMBL828799) Inhibition of [3H]prazosin binding to human adrenergic alpha-1A receptor expressed in CHO-K1 cell membranes
- ChEMBL_32400 (CHEMBL648036) Compound was tested for the inhibition of [3H]prazosin binding Alpha-1 adrenergic receptor of crude rat brain membrane.
- ChEMBL_32401 (CHEMBL648037) Compound was tested for the inhibition of [3H]prazosin binding alpha-1-adrenergic receptor of crude rat brain membrane.
- ChEMBL_32543 (CHEMBL646577) Inhibition of saturable binding of [3H]prazosin to alpha-1 adrenergic receptor site in rat cerebral cortical membranes.
- ChEMBL_32720 (CHEMBL645992) Displacement of [3H]prazosin (0.3 nM) from rat Alpha-1D adrenergic receptor expressed in CHO cells
- ChEMBL_33142 (CHEMBL642097) Binding affinity determined to alpha-1 adrenergic receptor of rat cerebral cortex using [3H]prazosin as radioligand
- ChEMBL_33143 (CHEMBL642098) Compound was evaluated for binding affinity towards Alpha-1 adrenergic receptor using [3H]prazosin in rat heart
- ChEMBL_33157 (CHEMBL642942) Ability to inhibit 50% specific binding of [3H]prazosin radioligand to alpha-1 adrenergic receptor in rat cerebrocortex
- ChEMBL_33276 (CHEMBL643614) Binding affinity for Alpha-1 adrenergic receptor measured by displacing [3H]prazosin from rat cortical membranes
- ChEMBL_33277 (CHEMBL644237) Binding affinity measured for alpha-1 adrenergic receptor by displacement of [3H]prazosin from rat cortical membranes
- ChEMBL_33428 (CHEMBL648824) Compound was measured in vivo for its binding affinity at Alpha-1 adrenergic receptor using [3H]prazosin as radioligand.
- ChEMBL_33572 (CHEMBL873485) Binding affinity at Alpha-1 adrenergic receptor sites on rat cerebral cortical membranes by [3H]prazosin displacement.
- ChEMBL_33577 (CHEMBL647128) Tested for binding affinity against alpha-1 adrenergic receptor in rat brain using [3H]-Prazosin as radioligand
- ChEMBL_33583 (CHEMBL647134) Affinity towards Alpha-1A adrenergic receptor in membranes from bovine frontal cortex using [3H]prazosin
- ChEMBL_33584 (CHEMBL647135) Displacement of [3H]prazosin (0.3 nM) from bovine Alpha-1A adrenergic receptor expressed in BHK cells
- ChEMBL_33721 (CHEMBL884034) Ability to inhibit 50% specific binding of [3H]prazosin radioligand to alpha-1 adrenergic receptor in rat cerebrocortex
- ChEMBL_33766 (CHEMBL651974) Compound was tested for the inhibition of [3H]prazosin binding Alpha-1 adrenergic receptor of crude rat brain membrane.
- ChEMBL_33767 (CHEMBL651975) Compound was tested for the inhibition of [3H]prazosin binding Alpha-2 adrenergic receptor of crude rat brain membrane.
- ChEMBL_34001 (CHEMBL643630) Inhibition of specific [3H]-prazosin binding (0.2 nM) to rat brain membranes alpha-1 adrenergic receptor
- ChEMBL_34025 (CHEMBL646014) Binding affinity towards Alpha-1A adrenergic receptor in rat hippocampal membranes using [3H]prazosin as radioligand
- ChEMBL_34320 (CHEMBL873168) Binding affinity towards Alpha-1B adrenergic receptor hamster smooth muscle using [3H]prazosin as radioligand
- ChEMBL_34432 (CHEMBL649435) Binding affinity for alpha-1 adrenergic receptor by displacement of [3H]-prazosin from rat brain homogenate preparation
- ChEMBL_34448 (CHEMBL651980) Binding affinity towards alpha-1 adrenergic receptor using [3H]prazosin as radioligand in competitive binding assay
- ChEMBL_34583 (CHEMBL645393) Binding affinity towards alpha-1 adrenergic receptor in rat cortex membrane using [3H]-prazosin radioligand
- ChEMBL_592535 (CHEMBL1046742) Displacement of [3H]prazosin from adrenergic alpha1A receptor in Wistar rat submaxillary gland after 60 mins
- ChEMBL_658237 (CHEMBL1246629) Displacement [3H]prazosin of human recombinant alpha1A adrenergic receptor expressed in CHO cells by rapid filtration technique
- ChEMBL_774870 (CHEMBL1913212) Displacement of [3H]prazosin from Alpha-1D receptor expressed in HEK293 EBNA cells by radioligand binding assay
- ChEMBL_774871 (CHEMBL1913213) Displacement of [3H]prazosin from Alpha-1B receptor expressed in HEK293 EBNA cells by radioligand binding assay
- ChEMBL_978685 (CHEMBL2422546) Displacement of [3H]prazosin from human alpha-1D adrenoreceptor expressed in CHO cells after 30 mins
- ChEMBL_978686 (CHEMBL2422547) Displacement of [3H]prazosin from human alpha-1B adrenoreceptor expressed in CHO cells after 30 mins
- ChEMBL_978687 (CHEMBL2422548) Displacement of [3H]prazosin from human alpha-1A adrenoreceptor expressed in CHO cells after 30 mins
- ChEBML_33095 Binding affinity against alpha-1 adrenergic receptor was determined in calf cerebral cortex homogenates using [3H]prazosin as radioligand
- ChEMBL_1625178 (CHEMBL3867647) Displacement of [3H]prazosin from human alpha1A-adrenoceptor expressed in CHO cell membranes measured after 30 mins
- ChEMBL_1625179 (CHEMBL3867648) Displacement of [3H]prazosin from human alpha1B-adrenoceptor expressed in CHO cell membranes measured after 30 mins
- ChEMBL_1625180 (CHEMBL3867649) Displacement of [3H]prazosin from human alpha1D-adrenoceptor expressed in CHO cell membranes measured after 30 mins
- ChEMBL_1627291 (CHEMBL3869812) Displacement of [3H]Prazosin from human Alpha1A receptor measured after 90 mins by microbeta scintillation counting method
- ChEMBL_1627293 (CHEMBL3869814) Displacement of [3H]Prazosin from human Alpha1D receptor measured after 90 mins by microbeta scintillation counting method
- ChEMBL_164467 (CHEMBL770733) Inhibition of specific [3H]-prazosin binding (0.2 nM) to rat brain membranes alpha1 adrenoceptor; 2.1*10e-6
- ChEMBL_1662837 (CHEMBL4012518) Displacement of [3H]prazosin from recombinant human adrenergic A1a receptor after 60 mins by scintillation counting method
- ChEMBL_1737641 (CHEMBL4153391) Displacement of [3H]-Prazosin from human adrenergic alpha1A receptor after 90 mins by microbeta scintillation counting method
- ChEMBL_1737645 (CHEMBL4153395) Displacement of [3H]-Prazosin from human adrenergic alpha1D receptor after 90 mins by microbeta scintillation counting method
- ChEMBL_1760119 (CHEMBL4195127) Displacement of [3H]-prazosin from Wistar rat cerebral cortex membranes alpha1 adrenoceptor by liquid scintillation counting method
- ChEMBL_1789086 (CHEMBL4260820) Displacement of [3H]Prazosin from human alpha 1D adrenergic receptor expressed in HEK cells by radioligand binding assay
- ChEMBL_1864030 (CHEMBL4365005) Displacement of [3H]prazosin from recombinant human alpha 1A adrenoceptor after 60 mins by scintillation counting analysis
- ChEMBL_2205189 (CHEMBL5117897) Displacement of [3H]prazosin from recombinant human alpha1A receptor measured after 60 mins by scintillation counting analysis
- ChEMBL_2205190 (CHEMBL5117898) Displacement of [3H]prazosin from recombinant human alpha1B receptor measured after 60 mins by scintillation counting analysis
- ChEMBL_2205191 (CHEMBL5117899) Displacement of [3H]prazosin from recombinant human alpha1D receptor measured after 60 mins by scintillation counting analysis
- ChEMBL_304055 (CHEMBL839353) Binding affinity towards human alpha-1B-adrenergic receptor expressed in CHO cells using the radioligand [3H]prazosin
- ChEMBL_32432 (CHEMBL873049) Binding affinity against human adrenergic receptor subtype Alpha-1D adrenergic receptor using [3H]prazosin as radioligand
- ChEMBL_32594 (CHEMBL857995) Binding affinity for human Alpha-1D adrenergic receptor from cultured LM(tk-) cells using [3H]- prazosin
- ChEMBL_32602 (CHEMBL642129) Inhibition of [3H]prazosin binding to CHO-K1 whole cells expressing human cloned Alpha-1D adrenergic receptor
- ChEMBL_32731 (CHEMBL646002) In vitro binding affinity using [3H]prazosin as radioligand against alpha-1D adrenergic receptor expressed in LTK cell
- ChEMBL_33034 (CHEMBL648049) Binding affinity against alpha-2 adrenergic receptor from calf cerebral cortex, using [3H]prazosin as the radioligand
- ChEMBL_33139 (CHEMBL642094) Binding affinity to alpha-1 adrenergic receptor determined by measurement of [3H]prazosin displacement from rat cortical membrane
- ChEMBL_33158 (CHEMBL642943) Binding affinity to the alpha-1 adrenergic receptor using a radioligand [3H]prazosin binding assay in rat brain membranes
- ChEMBL_33367 (CHEMBL645105) Binding affinity for human Alpha-2B adrenergic receptor from cultured LM(tk-) cells using [3H]- prazosin
- ChEMBL_33568 (CHEMBL648584) Alpha-1 adrenergic receptor binding affinity was evaluated by its ability to displace [3H]prazosin in rat brain synaptosomes
- ChEMBL_33607 (CHEMBL652814) Binding affinity against human adrenergic receptor subtype Alpha-1A adrenergic receptor using [3H]prazosin as radioligand
- ChEMBL_33664 (CHEMBL649245) Binding affinity for human Alpha-2C adrenergic receptor from cultured LM(tk-) cells using [3H]- prazosin
- ChEMBL_33835 (CHEMBL646864) Alpha-1-adrenolytic activity was assessed from the ability to inhibit [3H]prazosin binding to rat cerebral cortex preparation
- ChEMBL_33879 (CHEMBL643751) Binding affinity for human Alpha-1A adrenergic receptor from cultured LM(tk-) cells using [3H]-prazosin
- ChEMBL_33887 (CHEMBL643759) Inhibition of [3H]prazosin binding to CHO-K1 whole cells expressing human cloned Alpha-1A adrenergic receptor
- ChEMBL_34149 (CHEMBL644948) The concentration required to inhibit [3H]prazosin binding to Alpha-1 adrenergic receptor in rat brain membranes was measured (in vitro)
- ChEMBL_34197 (CHEMBL649214) Displacement of [3H]prazosin (0.5 nM) from hamster Alpha-1B adrenergic receptor expressed in rat-1 cells
- ChEMBL_34312 (CHEMBL648097) In vitro binding affinity using [3H]prazosin as radioligand against alpha-1B adrenergic receptor expressed in LTK cell
- ChEMBL_34330 (CHEMBL648113) Binding affinity against human adrenergic receptor subtype Alpha-1B adrenergic receptor using [3H]prazosin as radioligand
- ChEMBL_34444 (CHEMBL647024) Binding affinity towards Alpha-1 adrenergic receptor in rat cerebral cortex membranes using [3H]prazosin as radioligand
- ChEMBL_34451 (CHEMBL651983) Binding affinity determined against alpha-1 adrenergic receptor in rat cortex membranes using [3H]prazosin as radioligand
- ChEMBL_34490 (CHEMBL647202) Binding affinity for human Alpha-1B adrenergic receptor from cultured LM(tk-) cells using [3H]prazosin
- ChEMBL_34582 (CHEMBL645392) Binding affinity towards alpha-1 adrenergic receptor from rat brain homogenate preparation by displacement of [3H]prazosin
- ChEMBL_34616 (CHEMBL645574) Inhibition of [3H]prazosin binding to CHO-K1 whole cells expressing human cloned Alpha-1B adrenergic receptor
- ChEMBL_34632 (CHEMBL647277) Compound was tested for the tissue binding affinity using [3H]- Prazosin as radioligand to the rat liver Alpha-1B adrenergic receptor
- ChEMBL_34637 (CHEMBL647282) Binding affinity towards Alpha-1B adrenergic receptor native receptor in rat liver using [3H]prazosin as radioligand
- ChEMBL_500271 (CHEMBL969606) Displacement of [3H]prazosin from human adrenergic alpha1A receptor expressed in thymidine kinase-deficient mouse LM cells
- ChEMBL_591270 (CHEMBL1056908) Displacement of [3H]Prazosin from alpha1 adrenergic receptor in Wistar rat cerebral cortex membrane by liquid scintillation counting
- ChEMBL_592536 (CHEMBL1046743) Displacement of [3H]prazosin from adrenergic Alpha-1B receptor in Wistar rat liver membrane after 60 mins
- ChEMBL_658238 (CHEMBL1246630) Displacement [3H]prazosin of human recombinant Alpha-1B adrenergic receptor expressed in CHO cells by rapid filtration technique
- ChEMBL_658239 (CHEMBL1246631) Displacement [3H]prazosin of human recombinant Alpha-1D adrenergic receptor expressed in CHO cells by rapid filtration technique
- ChEMBL_823617 (CHEMBL2044618) Displacement of [3H]prazosin from alpha1 adrenoceptor in rat cerebral cortex after 30 mins by microbeta scintillation counting
- ChEMBL_828181 (CHEMBL2050113) Displacement of [3H]prazosin from alpha1 adrenoceptor in rat cerebral cortex after 30 mins by beta scintillation counting
- ChEMBL_852798 (CHEMBL2156105) Displacement of [3H]prazosin from rat liver adrenergic alpha1B receptor after 45 mins by liquid scintillation counter
- ChEMBL_970260 (CHEMBL2406663) Displacement of [3H]prazosin from alpha1-adrenergic receptor in rat cerebral cortex after 30 mins by scintillation counting
- ChEBML_32976 Compound was evaluated for binding affinity at bovine brain Alpha-1 adrenergic receptor in a filtration-based assay using [3H]prazosin as the radioligand
- ChEMBL_1499794 (CHEMBL3582624) Displacement of [3H]prazosin from human recombinant adrenergic alpha1B receptor expressed in HEKT cell membranes by radioligand binding assay
- ChEMBL_32296 (CHEMBL646243) Compound was tested for binding Alpha-1B adrenergic receptor from rat liver using radioligand ([3H]prazosin) binding assay
- ChEMBL_32410 (CHEMBL648045) In vitro binding affinity against Alpha-1 adrenergic receptor in rat cerebral cortex membrane using [3H]prazosin as radioligand
- ChEMBL_32418 (CHEMBL644957) In vitro binding affinity towards alpha-1 adrenergic receptor in rat frontal cortex homogenate using of [3H]prazosin as radioligand
- ChEMBL_32455 (CHEMBL643403) Inhibition of [3H]-prazosin binding to cloned human Alpha-1D adrenergic receptor in CHO cells (chinese hamster ovary cells)
- ChEMBL_32458 (CHEMBL643574) The compound was tested for binding affinity on [3H]- prazosin as specific ligand on Human cloned alpha-1D adrenergic receptor in CHO cells
- ChEMBL_33133 (CHEMBL642088) Binding affinity against Alpha-1 adrenergic receptor in rat cerebral cortical membrane, determined using [3H]-prazosin as the radioligand
- ChEMBL_33134 (CHEMBL642089) Binding affinity against alpha-1 adrenergic receptor in rat cerebral cortical membrane, determined using [3H]prazosin as the radioligand
- ChEMBL_33574 (CHEMBL647125) Displacement of [3H]prazosin binding to Alpha-1 adrenergic receptor sites on rat cerebral cortical membranes at 100 nM concentration
- ChEMBL_33600 (CHEMBL652808) Compound was tested for the tissue binding affinity using [3H]- Prazosin as radioligand to the human prostate Alpha-1A adrenergic receptor subtype
- ChEMBL_33601 (CHEMBL652809) Compound was tested for the tissue binding affinity using [3H]- Prazosin as radioligand to the human prostate Alpha-1A adrenergic receptor subtype.
- ChEMBL_33743 (CHEMBL647058) Inhibition of [3H]prazosin binding to cloned human Alpha-1A adrenergic receptor in CHO cells (chinese hamster ovary cells)
- ChEMBL_33841 (CHEMBL647615) Binding affinity by its ability to displace [3H]prazosin radioligand from Alpha-1 adrenergic receptor on rat cerebral cortex
- ChEMBL_33845 (CHEMBL649282) 50% inhibition of specific [3H]prazosin binding (0.4 nM) to Alpha-2 adrenergic receptors in rat isolated brain membranes
- ChEMBL_33864 (CHEMBL643737) Compound was tested for its inhibitory effect on the binding profile in Alpha-1 adrenergic receptor using [3H]prazosin in rat brain.
- ChEMBL_34013 (CHEMBL645380) Compound was tested for the tissue binding affinity using [3H]- Prazosin as radioligand to the rat prostate Alpha-1A adrenergic receptor subtype
- ChEMBL_34014 (CHEMBL645381) Compound was tested for the tissue binding affinity using [3H]- Prazosin as radioligand to the rat prostate Alpha-1A adrenergic receptor subtype.
- ChEMBL_34031 (CHEMBL646170) In vitro binding affinity towards alpha-1A adrenergic receptor through displacement of [3H]prazosin in epididymal rat vas deferens.
- ChEMBL_34148 (CHEMBL644947) The concentration required to inhibit 3[H]prazosin binding to Alpha-1 adrenergic receptor in rat brain membranes was measured (in vitro)
- ChEMBL_34209 (CHEMBL649226) Binding affinity against alpha-2 adrenergic receptor was determined by the displacement of [3H]prazosin from rat brain cortical membranes
- ChEMBL_34319 (CHEMBL648103) Binding affinity towards Alpha-1B adrenergic receptor cloned receptor hamster smooth muscle using [3H]prazosin as radioligand
- ChEMBL_34452 (CHEMBL651984) Binding affinity by its ability to displace [3H]prazosin radioligand from Alpha-1 adrenergic receptor on rat cerebral cortex
- ChEMBL_34461 (CHEMBL651993) Inhibition of [3H]prazosin binding to cloned human Alpha-1B adrenergic receptor in CHO cells (chinese hamster ovary cells)
- ChEMBL_34462 (CHEMBL651994) Inhibition of [3H]-prazosin binding to cloned human Alpha-1B adrenergic receptor in CHO cells (chinese hamster ovary cells)
- ChEMBL_34467 (CHEMBL651999) The compound was tested for binding affinity on [3H]- prazosin as specific ligand on Human cloned alpha-1B adrenergic receptor in CHO cells
- ChEMBL_34633 (CHEMBL647278) Compound was tested for the tissue binding affinity using [3H]- Prazosin as radioligand to the rat spleen Alpha-1B adrenergic receptor subtype
- ChEMBL_34634 (CHEMBL647279) Compound was tested for the tissue binding affinity using [3H]- Prazosin as radioligand to the rat spleen Alpha-1B adrenergic receptor subtype.
- ChEMBL_34641 (CHEMBL647286) Compound was tested for its binding affinity towards Alpha-1B adrenergic receptor using [3H]prazosin from rat liver
- ChEMBL_718125 (CHEMBL1671578) Displacement of [3H]-prazosin from alpha1-adrenergic receptor in rat cerebral cortex membranes after 30 mins by scintillation counting
- ChEMBL_936571 (CHEMBL2318734) Displacement of [3H]prazosin from human recombinant alpha1D adrenergic receptor expressed in CHO cell membranes after 30 mins
- ChEMBL_936572 (CHEMBL2318735) Displacement of [3H]prazosin from human recombinant alpha1B adrenergic receptor expressed in CHO cell membranes after 30 mins
- ChEMBL_936573 (CHEMBL2318736) Displacement of [3H]prazosin from human recombinant alpha1A adrenergic receptor expressed in CHO cell membranes after 30 mins
- ChEMBL_1440678 (CHEMBL3390430) Displacement of [3H]prazosin from human recombinant alpha1A adrenoceptor expressed in CHO cells after 30 mins by liquid scintillation counting
- ChEMBL_1440679 (CHEMBL3390431) Displacement of [3H]prazosin from human recombinant alpha1B adrenoceptor expressed in CHO cells after 30 mins by liquid scintillation counting
- ChEMBL_1440680 (CHEMBL3381315) Displacement of [3H]prazosin from human recombinant alpha1D adrenoceptor expressed in CHO cells after 30 mins by liquid scintillation counting
- ChEMBL_1442353 (CHEMBL3378311) Displacement of [3H]prazosin from alpha1-adrenoceptor in Sprague-Dawley rat brain membranes after 45 mins by liquid scintillation counting
- ChEMBL_1732884 (CHEMBL4148420) Displacement of [3H]-prazosin from alpha1-adrenergic receptor in rat brain cortex after 30 mins by Microbeta scintillation counting method
- ChEMBL_1855931 (CHEMBL4356660) Displacement of [3H]-prazosin from human alpha1A adrenoreceptor expressed in enriched membranes incubated for 90 mins by liquid scintillation counting method
- ChEMBL_1878691 (CHEMBL4380085) Displacement of [3H]prazosin from human recombinant adrenergic alpha-1A receptor expressed in CHO cell membranes incubated for 60 mins
- ChEMBL_1878692 (CHEMBL4380086) Displacement of [3H]prazosin from human recombinant adrenergic alpha-1B receptor expressed in CHO cell membranes incubated for 60 mins
- ChEMBL_1878693 (CHEMBL4380087) Displacement of [3H]prazosin from human recombinant adrenergic alpha-1D receptor expressed in CHO cell membranes incubated for 60 mins
- ChEMBL_1890392 (CHEMBL4392146) Displacement of [3H]-prazosin from recombinant human alpha1A adrenergic receptor measured after 90 mins by microbeta scintillation counting method
- ChEMBL_1895367 (CHEMBL4397402) Displacement of [3H]-prazosin from recombinant human alpha1A adrenergic receptor measured after 90 mins by microbeta scintillation counting method
- ChEMBL_1904389 (CHEMBL4406611) Displacement of [3H]-prazosin from recombinant human alpha1A adrenergic receptor measured after 90 mins by microbeta scintillation counting method
- ChEMBL_1904391 (CHEMBL4406613) Displacement of [3H]-prazosin from recombinant human alpha1D adrenergic receptor measured after 90 mins by microbeta scintillation counting method
- ChEMBL_2052367 (CHEMBL4707368) Displacement of [3H]-prazosin from alpha1 adrenergic receptor in rat cortex measured after 30 mins by microbeta2 scintillation counting method
- ChEMBL_2361429 Displacement of [3H] prazosin from human recombinant adrenergic alpha-1d receptor expressed in HEK293 cells incubated for 60 mins by spectrophotometric analysis
- ChEMBL_32392 (CHEMBL648028) Compound was evaluated for the binding affinity towards Alpha-1 adrenergic receptor from rat whole brain using [3H]prazosin as radioligand
- ChEMBL_32600 (CHEMBL640173) Compound was tested for binding affinity against human hippocampus Alpha-1D adrenergic receptor using radioligand ([3H]prazosin) binding assay
- ChEMBL_33283 (CHEMBL644243) Binding affinities against Alpha-1 adrenergic receptor (alpha1 adrenoceptor) using [3H]prazosin as radioligand in rat cerebral cortex membrane
- ChEMBL_33748 (CHEMBL647825) The compound was tested for binding affinity on [3H]- prazosin as specific ligand on Human cloned alpha-1A adrenergic receptor expressed in CHO cells
- ChEMBL_33885 (CHEMBL643757) Compound was tested for binding affinity against human hippocampus Alpha-1A adrenergic receptor using radioligand ([3H]prazosin) binding assay
- ChEMBL_34024 (CHEMBL646013) Binding affinity towards Alpha-1A adrenergic receptor in rat hippocampus (+ 10 uM) CES membrane using [3H]prazosin as radioligand
- ChEMBL_34029 (CHEMBL646168) Compound was tested for its binding affinity towards Alpha-1A adrenergic receptor using [3H]prazosin from rat submaxillary gland
- ChEMBL_34425 (CHEMBL649429) Binding affinity against alpha-1 adrenergic receptor by measuring displacement of [3H]prazosin from rat brain cortex membranes (in vitro).
- ChEMBL_34613 (CHEMBL645571) Compound was tested for binding affinity against human hippocampus Alpha-1B adrenergic receptor using radioligand ([3H]prazosin) binding assay
- ChEMBL_34614 (CHEMBL645572) Compound was tested for binding affinity against human hippocampus Alpha-1B adrenergic receptor using radioligand ([3H]-prazosin) binding assay
- ChEMBL_800453 (CHEMBL1948474) Displacement of [3H]prazosin from human recombinant alpha1A adrenoceptor expressed in CHO cells after 30 mins by liquid scintillation counting
- ChEMBL_800454 (CHEMBL1948475) Displacement of [3H]prazosin from human recombinant alpha1B adrenoceptor expressed in CHO cells after 30 mins by liquid scintillation counting
- ChEMBL_852797 (CHEMBL2156104) Displacement of [3H]prazosin from rat salivary gland adrenergic alpha 1A receptor after 45 mins by liquid scintillation counter
- ChEMBL_947077 (CHEMBL2340582) Displacement of [3H]-prazosin from alpha1B adrenergic receptor in Wistar rat liver membrane after 45 mins by liquid scintillation counting
- ChEMBL_1709546 (CHEMBL4119595) Displacement of [3H]prazosin from alpha1 adrenoceptor in C57BL/6 mouse hippocampal homogenates after 40 mins by liquid scintillation counting method
- ChEMBL_1713733 (CHEMBL4123782) Displacement of [3H]prazosin from human alpha1A adrenergic receptor expressed in CHO cell membranes after 30 mins by rapid filtration method
- ChEMBL_1713734 (CHEMBL4123783) Displacement of [3H]prazosin from human alpha1B adrenergic receptor expressed in CHO cell membranes after 30 mins by rapid filtration method
- ChEMBL_1713735 (CHEMBL4123784) Displacement of [3H]prazosin from human alpha1D adrenergic receptor expressed in CHO cell membranes after 30 mins by rapid filtration method
- ChEMBL_1737643 (CHEMBL4153393) Displacement of [3H]-Prazosin from human adrenergic alpha1B receptor expressed in HEKT cells after 90 mins by microbeta scintillation counting method
- ChEMBL_1783042 (CHEMBL4254559) Displacement of [3H]prazosin from alpha1-adrenoceptor (unknown origin) in cerebral cortex membranes after 60 mins by TopCount scintillation counting method
- ChEMBL_1804293 (CHEMBL4276585) Displacement of [3H]-prazosin from alpha-1 adrenergic receptor in rat cerebral cortex homogenates incubated for 60 mins by liquid scintillation counting
- ChEMBL_1809256 (CHEMBL4308615) Displacement of [3H]-Prazosin from human recombinant adrenergic alpha1A receptor expressed in cells after 90 mins by microbeta scintillation counting method
- ChEMBL_1809258 (CHEMBL4308617) Displacement of [3H]-Prazosin from human recombinant adrenergic Alpha1D receptor expressed in cells after 90 mins by microbeta scintillation counting method
- ChEMBL_2016820 (CHEMBL4670398) Displacement of [3H]prazosin from alpha1 adrenergic receptor in rat cortex membranes measured after 30 mins by Microbeta2 scintillation counting method
- ChEMBL_2054842 (CHEMBL4709843) Displacement of [3H]prazosin from rat cerebral cortex alpha1 adrenergic receptor measured after 60 mins by liquid scintillation counting method
- ChEMBL_2340782 Displacement of [3H]prazosin from adrenergic alpha 1A receptor (unknown origin) expressed in HEK293T cells assessed as inhibition constant by competition binding assay
- ChEMBL_32975 (CHEMBL644690) Compound was evaluated for binding affinity at bovine brain Alpha-1 adrenergic receptor determined in a filtration-based assay using [3H]prazosin as the radioligand.
- ChEMBL_33097 (CHEMBL645100) Binding affinity of compound towards Alpha-1 adrenergic receptor using [3H]prazosin 0.5 nM ligand in frontal cortex calf was determined
- ChEMBL_33135 (CHEMBL642090) Binding affinity against native Alpha-1 adrenergic receptor from rat cerebral cortex using radioligand [3H]-prazosin binding assay
- ChEMBL_34600 (CHEMBL645559) Alpha-1 adrenergic receptor binding affinity by measuring the displacement of [3H]-prazosin binding in rat brain; Not Active means Ki >1000 nM
- ChEMBL_664774 (CHEMBL1259384) Displacement of [3H]-prazosin from human adrenergic alpha-1b receptor expressed in CHO cells after 120 mins by liquid scintillation counting
- ChEMBL_800455 (CHEMBL1948476) Displacement of [3H]prazosin from human recombinant Alpha-1D adrenoceptor expressed in CHO cells after 30 mins by liquid scintillation counting
- ChEMBL_854041 (CHEMBL2155338) Displacement of [3H]prazosin from human cloned alpha1B adrenoceptor expressed in CHO cell membranes after 30 mins by liquid scintillation counter
- ChEMBL_854042 (CHEMBL2155339) Displacement of [3H]prazosin from human cloned alpha1A adrenoceptor expressed in CHO cell membranes after 30 mins by liquid scintillation counter
- ChEMBL_854044 (CHEMBL2155385) Displacement of [3H]prazosin from human cloned alpha1D adrenoceptor expressed in CHO cell membranes after 30 mins by liquid scintillation counter
- ChEMBL_1627292 (CHEMBL3869813) Displacement of [3H]Prazosin from human Alpha1B receptor expressed in HEK-T cells measured after 90 mins by microbeta scintillation counting method
- ChEMBL_1712279 (CHEMBL4122328) Displacement of [3H]8-prazosin from adrenergic alpha1 receptor in Wistar rat cortex incubated for 30 mins by microbeta liquid scintillation counting analysis
- ChEMBL_1760129 (CHEMBL4195137) Displacement of [3H]prazosin from recombinant human alpha1 adrenoceptor expressed in CHO cell membranes after 30 mins by liquid scintillation counting method
- ChEMBL_1809257 (CHEMBL4308616) Displacement of [3H]-Prazosin from human recombinant adrenergic Alpha1B receptor expressed in HEKT cells after 90 mins by microbeta scintillation counting method
- ChEMBL_1852731 (CHEMBL4353355) Displacement of [3H] prazosin from human cloned adrenoceptor alpha 1A expressed in CHO cell membrane incubated for 30 mins by liquid scintillation counting
- ChEMBL_1852732 (CHEMBL4353356) Displacement of [3H] prazosin from human cloned adrenoceptor alpha 1B expressed in CHO cell membrane incubated for 30 mins by liquid scintillation counter
- ChEMBL_1852733 (CHEMBL4353357) Displacement of [3H] prazosin from human cloned adrenoceptor alpha 1D expressed in CHO cell membrane incubated for 30 mins by liquid scintillation counter
- ChEMBL_2361427 Displacement of [3H] prazosin from human recombinant adrenergic alpha-1a receptor expressed in human Chem-1 cells incubated for 60 mins by spectrophotometric analysis
- ChEMBL_2361428 Displacement of [3H] prazosin from human recombinant adrenergic alpha-1b receptor expressed in human Chem-1 cells incubated for 60 mins by spectrophotometric analysis
- ChEMBL_32297 (CHEMBL646244) Compound was tested for binding affinity against native Alpha-1B adrenergic receptor from rat liver using radioligand ([3H]prazosin) binding assay
- ChEMBL_32393 (CHEMBL648029) Binding affinity towards Alpha-1 adrenergic receptor from rat whole brain using [3H]prazosin (18.1 Ci/mmol,0.9nanoM)as radioligand
- ChEMBL_32604 (CHEMBL642131) The binding affinity was evaluated on cloned human Alpha-1D adrenergic receptor expressed in chinese hamster ovary(CHO) cells by using [3H]-prazosin as radioligand.
- ChEMBL_32712 (CHEMBL644030) The binding affinity was evaluated on cloned human alpha-1D adrenergic receptor expressed in chinese hamster ovary(CHO) cells by using [3H]prazosin as radioligand.
- ChEMBL_32869 (CHEMBL648009) Compound was tested for binding affinity against cloned Alpha-1D adrenergic receptor from rat brain using radioligand ([3H]prazosin) binding assay
- ChEMBL_33593 (CHEMBL652802) Compound was tested for binding affinity against cloned Alpha-1A adrenergic receptor from bovine brain using radioligand ([3H]prazosin) binding assay
- ChEMBL_33889 (CHEMBL645467) The binding affinity was evaluated on cloned human Alpha-1A adrenergic receptor expressed in chinese hamster ovary(CHO) cells by using [3H]prazosin as radioligand.
- ChEMBL_33890 (CHEMBL645468) The binding affinity was evaluated on cloned human alpha-1A adrenergic receptor expressed in chinese hamster ovary(CHO) cells by using [3H]prazosin as radioligand.
- ChEMBL_34020 (CHEMBL646009) Binding affinity measured at the Alpha-1A adrenergic receptor by the inhibition of [3H]prazosin binding to rat cortex using unlabeled WB-4101 for nonspecific binding.
- ChEMBL_34173 (CHEMBL857770) Binding affinity against native Alpha-1A adrenergic receptor from rat hippocampus pretreated with chloroethylclonidine using radioligand ([3H]prazosin) binding assay
- ChEMBL_34578 (CHEMBL649340) Binding affinity towards Alpha-1 adrenergic receptor via standard competitive displacement assay using rat brain homogenate with [3H]prazosin as radioligand
- ChEMBL_34580 (CHEMBL645477) Binding affinity towards Alpha-1 adrenergic receptor was determined by competitive displacement assays using rat brain homogenate with [3H]prazosin as the competitive ligand
- ChEMBL_34621 (CHEMBL643902) The binding affinity was evaluated on cloned human Alpha-1B adrenergic receptor expressed in chinese hamster ovary(CHO) cells by using [3H]prazosin as radioligand.
- ChEMBL_34622 (CHEMBL643903) The binding affinity was evaluated on cloned human alpha-1B adrenergic receptor expressed in chinese hamster ovary(CHO) cells by using [3H]prazosin as radioligand.
- ChEBML_1691073 Displacement of [3H]prazosin from recombinant human alpha-1b adrenergic receptor expressed in CHO cell membranes after 30 mins by liquid scintillation counting method
- ChEBML_1691074 Displacement of [3H]prazosin from recombinant human alpha-1a adrenergic receptor expressed in CHO cell membranes after 30 mins by liquid scintillation counting method
- ChEBML_1691075 Displacement of [3H]prazosin from recombinant human alpha-1d adrenergic receptor expressed in CHO cell membranes after 30 mins by liquid scintillation counting method
- ChEBML_1698004 Displacement of [3H]prazosin from human recombinant alpha-1A adrenergic receptor expressed in CHO cell membranes after 30 mins by liquid scintillation counting method
- ChEBML_1698005 Displacement of [3H]prazosin from human recombinant alpha-1B adrenergic receptor expressed in CHO cell membranes after 30 mins by liquid scintillation counting method
- ChEBML_1698006 Displacement of [3H]prazosin from human recombinant alpha-1D adrenergic receptor expressed in CHO cell membranes after 30 mins by liquid scintillation counting method
- ChEMBL_1497770 (CHEMBL3583290) Displacement of [3H]-Prazosin from human alpha-1A adrenergic receptor transfected in CHO cell membranes after 2 hrs by microplate scintillation counting analysis
- ChEMBL_1497771 (CHEMBL3583291) Displacement of [3H]-Prazosin from human alpha-1B adrenergic receptor transfected in CHO cell membranes after 2 hrs by microplate scintillation counting analysis
- ChEMBL_1497913 (CHEMBL3583057) Displacement of [3H]-Prazosin from human alpha-1D adrenergic receptor transfected in CHO cell membranes after 2 hrs by microplate scintillation counting analysis
- ChEMBL_1890158 (CHEMBL4391912) Displacement of [3H]Prazosin from human recombinant alpha 1A adrenergic receptor expressed in CHO cells incubated for 30 mins by radioligand competition binding assay
- ChEMBL_1890159 (CHEMBL4391913) Displacement of [3H]Prazosin from human recombinant alpha 1B adrenergic receptor expressed in CHO cells incubated for 30 mins by radioligand competition binding assay
- ChEMBL_1890160 (CHEMBL4391914) Displacement of [3H]Prazosin from human recombinant alpha 1D adrenergic receptor expressed in CHO cells incubated for 30 mins by radioligand competition binding assay
- ChEMBL_2215574 (CHEMBL5128706) Inhibition of ABCG2 (unknown origin) expressed in human HEK293 cells assessed as BODIPY-Prazosin uptake and measured after 2 hrs by FACScan flow cytometry analysis
- ChEMBL_2237597 (CHEMBL5151493) Displacement of [3H]prazosin from human alpha-1A adrenergic receptor stably expressed in HEK293T cell membrane incubated for 2 hrs by scintillation counting analysis
- ChEMBL_2361447 Displacement of [3H] prazosin from human recombinant adrenergic alpha-1d receptor expressed in HEK293 cells assessed as inhibition constant incubated for 60 mins by spectrophotometric analysis
- ChEMBL_32407 (CHEMBL872271) In vitro ability to displace Alpha-1 adrenergic receptor binding to rat cerebral cortex in competition experiments against [3H]prazosin at 15mg/kg, expressed as Ki.
- ChEMBL_33573 (CHEMBL648588) Compound is evaluated for its ability to compete with [3H]prazosin for binding to Alpha-1 adrenergic receptor sites on rat cerebral cortical membranes at 1000 nM concentration
- ChEMBL_33575 (CHEMBL647126) Compound is evaluated for its ability to compete with [3H]prazosin for binding to Alpha-1 adrenergic receptor sites on rat cerebral cortical membranes at 10 nM concentration
- ChEMBL_33689 (CHEMBL873484) Compound is evaluated for its ability to compete with [3H]prazosin for binding to Alpha-1 adrenergic receptor sites on rat cerebral cortical membranes at 100 nM concentration
- ChEMBL_34317 (CHEMBL648101) Compound was tested for binding affinity against cloned Alpha-1B adrenergic receptor from hamster smooth muscle using radioligand ([3H]prazosin) binding assay
- ChEBML_1575788 Displacement of 7-methoxy-[3H]-prazosin from human alpha1D adrenergic receptor expressed in CHO-K1 cell membranes incubated for 60 mins by liquid scintillation counting analysis
- ChEMBL_1635128 (CHEMBL3878026) Displacement of [3H]prazosin from recombinant human alpha1a adrenergic receptor expressed in CHO cell membranes after 30 mins by TopCount liquid scintillation counting method
- ChEMBL_1635129 (CHEMBL3878027) Displacement of [3H]prazosin from recombinant human alpha1b adrenergic receptor expressed in CHO cell membranes after 30 mins by TopCount liquid scintillation counting method
- ChEMBL_1635130 (CHEMBL3878028) Displacement of [3H]prazosin from recombinant human alpha1d adrenergic receptor expressed in CHO cell membranes after 30 mins by TopCount liquid scintillation counting method
- ChEMBL_1758331 (CHEMBL4193339) Displacement of 7-methoxy-[3H]-prazosin from human alpha1D-adrenoreceptor expressed in CHOK1 cell membranes after 60 mins by TopCount liquid scintillation counting method
- ChEMBL_1758332 (CHEMBL4193340) Displacement of 7-methoxy-[3H]-prazosin from human alpha1B-adrenoreceptor expressed in CHOK1 cell membranes after 60 mins by TopCount liquid scintillation counting method
- ChEMBL_1758333 (CHEMBL4193341) Displacement of 7-methoxy-[3H]-prazosin from human alpha1A-adrenoreceptor expressed in CHOK1 cell membranes after 60 mins by TopCount liquid scintillation counting method
- ChEMBL_1890393 (CHEMBL4392147) Displacement of [3H]-prazosin from recombinant human alpha1B adrenergic receptor transiently expressed in HEKT cells measured after 90 mins by microbeta scintillation counting method
- ChEMBL_1895368 (CHEMBL4397403) Displacement of [3H]-prazosin from recombinant human alpha1B adrenergic receptor transiently expressed in HEKT cells measured after 90 mins by microbeta scintillation counting method
- ChEMBL_32408 (CHEMBL648043) In vitro ability to displace Alpha-1 adrenergic receptor binding to rat cerebral cortex in competition experiments against [3H]-prazosin at 1 mg/kg, expressed as Ki.
- ChEMBL_33844 (CHEMBL649281) Binding affinity against alpha-1 adrenergic receptor is the ability to inhibit the specific [3H]prazosin binding (0.4 nM) to rat isolated brain membranes by 50% was reported.
- ChEMBL_34426 (CHEMBL873184) Binding affinity against alpha-1 adrenergic receptor was determined by the displacement of [3H]prazosin from rat brain cortical membranes; value ranges from 221-499
- ChEMBL_34427 (CHEMBL649430) Binding affinity against alpha-1 adrenergic receptor was determined by the displacement of [3H]prazosin from rat brain cortical membranes; value ranges from 4.6-30.6
- ChEMBL_34428 (CHEMBL649431) Binding affinity against alpha-1 adrenergic receptor was determined by the displacement of [3H]prazosin from rat brain cortical membranes; value ranges from 6.06-7.06
- ChEMBL_34429 (CHEMBL649432) Binding affinity against alpha-1 adrenergic receptor was determined by the displacement of [3H]prazosin from rat brain cortical membranes; value ranges from 7.8-34.6
- ChEMBL_1575788 (CHEMBL3801620) Displacement of 7-methoxy-[3H]-prazosin from human alpha1D adrenergic receptor expressed in CHO-K1 cell membranes incubated for 60 mins by liquid scintillation counting analysis
- ChEMBL_1575805 (CHEMBL3801637) Displacement of 7-methoxy-[3H]-prazosin from human alpha1A adrenergic receptor expressed in CHO-K1 cell membranes incubated for 60 mins by liquid scintillation counting analysis
- ChEMBL_1575806 (CHEMBL3801638) Displacement of 7-methoxy-[3H]-prazosin from human alpha1B adrenergic receptor expressed in CHO-K1 cell membranes incubated for 60 mins by liquid scintillation counting analysis
- ChEMBL_1904390 (CHEMBL4406612) Displacement of [3H]-prazosin from recombinant human alpha1B adrenergic receptor transiently expressed in HEKT cell membranes measured after 90 mins by microbeta scintillation counting method
- ChEMBL_2361445 Displacement of [3H] prazosin from human recombinant adrenergic alpha-1a receptor expressed in human Chem-1 cells assessed as inhibition constant incubated for 60 mins by spectrophotometric analysis
- ChEMBL_2361446 Displacement of [3H] prazosin from human recombinant adrenergic alpha-1b receptor expressed in human Chem-1 cells assessed as inhibition constant incubated for 60 mins by spectrophotometric analysis
- ChEMBL_33846 (CHEMBL649283) Binding affinity against alpha-1 adrenergic receptor is the ability to inhibit the specific [3H]prazosin binding (0.2 nM) to rat isolated brain membranes by 50% was reported; 1.6*10e-6
- ChEMBL_33847 (CHEMBL649284) Binding affinity against alpha-1 adrenergic receptor is the ability to inhibit the specific [3H]prazosin binding (0.2 nM) to rat isolated brain membranes by 50% was reported; 2.4*10e-6
- ChEMBL_33848 (CHEMBL649285) Binding affinity against alpha-1 adrenergic receptor is the ability to inhibit the specific [3H]prazosin binding (0.2 nM) to rat isolated brain membranes by 50% was reported; 4.1*10e-7
- ChEMBL_33849 (CHEMBL649286) Binding affinity against alpha-1 adrenergic receptor is the ability to inhibit the specific [3H]-prazosin binding (0.2 nM) to rat isolated brain membranes by 50% was reported; 4.85*10e-6
- ChEMBL_33850 (CHEMBL649287) Binding affinity against alpha-1 adrenergic receptor is the ability to inhibit the specific [3H]-prazosin binding (0.2 nM) to rat isolated brain membranes by 50% was reported; 5*10e-4
- ChEMBL_33851 (CHEMBL649288) Binding affinity against alpha-1 adrenergic receptor is the ability to inhibit the specific [3H]prazosin binding (0.2 nM) to rat isolated brain membranes by 50% was reported; 5.8*10e-7
- ChEMBL_33852 (CHEMBL649289) Binding affinity against alpha-1 adrenergic receptor is the ability to inhibit the specific [3H]prazosin binding (0.2 nM) to rat isolated brain membranes by 50% was reported; 6.1*10e-5
- ChEMBL_33853 (CHEMBL649290) Binding affinity against alpha-1 adrenergic receptor is the ability to inhibit the specific [3H]prazosin binding (0.2 nM) to rat isolated brain membranes by 50% was reported; 6.5*10e-5
- ChEMBL_33854 (CHEMBL649291) Binding affinity against alpha-1 adrenergic receptor is the ability to inhibit the specific [3H]prazosin binding (0.2 nM) to rat isolated brain membranes by 50% was reported; 6.6*10e-7
- ChEMBL_33855 (CHEMBL649292) Binding affinity against alpha-1 adrenergic receptor is the ability to inhibit the specific [3H]prazosin binding (0.2 nM) to rat isolated brain membranes by 50% was reported; 8.9*10e-7
- ChEMBL_2215590 (CHEMBL5128722) Inhibition of ABCG2 (unknown origin) expressed in human MCF7/MX100 cells assessed as BODIPY-Prazosin uptake and preincubated with substrate followed by compound addition and measured after 1 hrs by flow cytometry analysis
- Binding Assay Binding to the rat alpha-1 adrenergic receptor in rat brain membranes was determined by displacement of 3[H]-prazosin (P. Greengrass and R. Bremner; Binding characteristics of 3H-prazosin to rat brain a-adrenergic receptors. Eur. J. Pharmacol. 1979, 55: 323-326). Compounds were incubated at 0.3 or 3 μM final concentration, in duplicate, and the amount of displaced 3[H]-prazosin determined by liquid scintillation spectroscopy.Binding IC50 values were determined from displacement curves (four-six concentrations, each concentration in duplicate) fit by a non-linear, least squares, regression analysis using MathIQ (ID Business Solutions Ltd., UK). The binding Ki's were determined from the IC50 according to the method of Cheng and Prusoff (Y. Cheng and W. H. Prusoff, Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 percent inhibition (IC50) of an enzymatic reaction. Biochem. Pharmacol. 1973, 22: 3099-3108).
- Binding Assay To prepare cell membranes with human α1- and α2-adrenergic receptors, CHO cells stably overexpressing α1- and α2-adrenergic receptors are lysed and then subjected to differential centrifugation. After lysis in binding buffer (50 mM tris(hydroxymethyl)aminomethane/1 N hydrochloric acid, 5 mM magnesium chloride, pH 7.4) using an Ultra Turrax (Jahnke & Kunkel, Werk), the homogenate is centrifuged at 1000 g and at 4° C. for 10 min. The resulting sediment is discarded and the supernatant is centrifuged at 20 000 g and at 4° C. for 30 min. The supernatant is discarded and the sediment is resuspended in binding buffer and stored at −70° C. until the binding test. For the binding test the radioligands 3H-MK-912 (2.2-3.2 TBq/mmol, PerkinElmer) (0.4 nM for α2C-adrRez and 1 nM for α2A-adrRez), 0.25 nM 3H-prazosin (α1AC-adrRez; 2.6-3.3 TBq/mmol, PerkinElmer), 0.25 nM 3H-rauwolscine (α2B-adrRez, 2.6-3.2 TBq/mmol, PerkinElmer) are incubated for 60 minutes with 5-20 μg cell membranes in binding buffer (total test volume 0.2 ml) in the presence of the test substances at 30° C. in 96-well filter plates (FC/B glass fibre, Multiscreen Millipore). The incubating is terminated by aspiration of the unbound radioactivity and the plates are then washed with binding buffer and subsequently dried at 40° C. for 1 hour. Liquid scintillator (Ultima Gold, PerkinElmer) is then added and the radioactivity that remained on the plates is measured in a liquid scintillation counter (Microbeta, Wallac). Non-specific binding is defined as radioactivity in the presence of 1-10 μM WB-4101 (α2C-adrRez and α2A-adrRez), prazosin (α2B-adrRez and α1AC-adrRez) (all from Sigma) and is generally <25% of the bound total radioactivity.
- In Vitro Radioligand Binding Assay In Vitro Radioligand Binding Studies for Determination of the Dissociation Constants K1 at the Human Adrenoreceptor ADRA2C (Eurofins Panlabs Discovery Services, Taiwan, Ltd)A competition assay based on [3H] rauwolscine as radioliganden was used to determine the binding affinity of the test substances at the human ADRA2C receptor.To configure the competition assay, the equilibrium dissociation constant Kd of the radioligand [3H] rauwolscine was determined in a saturation experiment. To this end, homogenates of CHO-K1 cells recombinantly expressing the human ADRA2C receptor were incubated with increasing concentrations of the radiotracers for 1 h at 4° C. in binding buffer (50 mM Tris-HCl, 1 mM EDTA, pH 7.4). Unspecific binding was determined by addition of an excess of the not radioactively labelled ligand prazosin (10 μM). The radioactivity was determined in a scintiation counter.The competition experiments were carried out in the presence of 0.5 nM [3H] rauwolscine and increasing concentrations of the test substances to be characterized under the conditions described above. The substance concentration which displaces 50% of the radiolabelled ligand is referred to as IC50 value.
- Radioligand Binding Assay Alpha1-adrenergic Receptor: Radioligand binding was performed using tissue (rat cortex). All assays were carried out in duplicates. 50 μL working solution of the tested compounds, 50 μL [3H]-prazosin (final concentration 0.2 nM, Kd 0.2 nM) and 150 μL tissue suspension prepared in assay buffer (50 mM Tris-HCl, pH 7.6) were transferred to polypropylene 96well microplate using 96wells pipetting station Rainin Liquidator (MettlerToledo). Phentolamine (10 μM) was used to define nonspecific binding. Microplate was covered with a sealing tape, mixed and incubated for 30 minutes at 30° C. The reaction was terminated by rapid filtration through GF/B filter mate. Ten rapid washes with 200 μL 50 mM Tris-HCl buffer (4° C., pH 7.6) were performed using automated harvester system Harvester-96 MACH III FM (Tomtec). The filter mates were dried at 37° C. in forced air fan incubator and soaked in 10 mL of liquid scintillation cocktail Ultima Gold MV (PerkinElmer, USA). After even distribution of scintillation cocktail filter bag was sealed. Radioactivity was counted in MicroBeta2 scintillation counter (PerkinElmer) at approximately 30% efficiency. Data were fitted to a one-site curve-fitting equation with Prism 6 (Graph Pad Software) and Ki values were estimated from the Cheng-Prusoff equation.