Query String: SELEGILINE
Selegiline BDBM15579 US8633208, Deprenyl methyl[(2R)-1-phenylpropan-2-yl]prop-2-yn-1-ylamine DEPRENYL CHEMBL972 US9469653, Selegiline L-Deprenyl N-methyl-N-[(2R)-1-phenylpropan-2-yl]prop-2-yn-1-amine SLG
Selegiline methyl-(1-methyl-2-phenyl-ethyl)-propargyl-amine;hydrochloride N-methyl-1-phenyl-N-prop-2-ynyl-2-propanamine;hydrochloride Deprenyl MLS000069378 cid_92913 N-methyl-1-phenyl-N-prop-2-ynylpropan-2-amine;hydrochloride METHYL-(1-METHYL-2-PHENYL-ETHYL)-PROP-2-YNYL-AMINE HYDROCHLORIDE N-methyl-1-phenyl-N-prop-2-ynyl-propan-2-amine;hydrochloride BDBM39862 SMR000058408
- Lu, C; Zhou, Q; Yan, J; Du, Z; Huang, L; Li, X A novel series of tacrine-selegiline hybrids with cholinesterase and monoamine oxidase inhibition activities for the treatment of Alzheimer's disease. Eur J Med Chem 62: 745-53 (2013)
- Xie, S; Chen, J; Li, X; Su, T; Wang, Y; Wang, Z; Huang, L; Li, X Synthesis and evaluation of selegiline derivatives as monoamine oxidase inhibitor, antioxidant and metal chelator against Alzheimer's disease. Bioorg Med Chem 23: 3722-9 (2015)
- ChEMBL_1464354 (CHEMBL3404813) Inhibition of MAO-A in rat whole brain homogenate in presence of selegiline
- ChEMBL_1647765 (CHEMBL3996821) Inhibition of MAOA in Sprague-Dawley rat liver mitochondria using p-tyramine as substrate measured for 45 mins in presence of MAOB inhibitor selegiline by continuous fluorescence-based method
- ChEMBL_1647766 (CHEMBL3996822) Inhibition of recombinant human MAOA expressed in baculovirus-infected BTI insect cells using p-tyramine as substrate measured for 45 mins in presence of MAOB inhibitor selegiline by continuous fluorescence-based method
- ChEMBL_2261432 (CHEMBL5216443) Displacement of 14C-5-hydroxytryptamine creatinine disulfate from MAO-A (unknown origin) preincubated for 60 mins followed by substrate addition measured after 30 mins in presence of selegiline by liquid scintillation counting method
- MAO Inhibition Assay A continuous spectrophotometric assay that monitors the rate of oxidation of the nonselective MAO substrate kynuramine into 4-hydroxyquinoline was used. clorgyline or selegiline was preincubated with the suspension for 5 min to measure MAO-B or MAO-A activity, respectively. The formation of 4-hydroxyquinoline was followed at 314 nm using a Kontron UVIKON 941 spectrophotometer. IC50 values were determined and calculated from a hyperbolic equation. Each assay was performed in triplicate on four to six separate homogenates.
- Fluorimetric Assay The activities of recombinant hMAO-A and hMAO-B were determined using p-tyramine as common substrate and calculated as 0.18 +/- 0.01 nmol/mg/min (n = 3) and 0.12 +/- 0.02 nmol/mg/min (n = 3), respectively. The interactions of the synthesized compounds with hMAO isoforms were determined by a fluorimetric method described previously. The production of H2O2 catalyzed by MAO isoforms was detected using a non-fluorescent Amplex-Red reagent which reacts with H2O2 in the presence of horseradish peroxidase to produce the fluorescent product resorufin. The reaction was started by the addition of 200 uM Amplex Red reagent, 1 U/mL horseradish peroxidase, and p-tyramine (concentration range 0.1 to 1 mM). Control experiments were carried out using reference inhibitors (Selegiline and Moclobemide). The possible capacity of compounds to modify the fluorescence generated in the reaction mixture due to non-enzymatic inhibition was determined by adding these compounds to solutions containing only the Amplex Red reagent in sodium phosphate buffer.