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Query String: TRIPROLIDINE

BDBM86174 Triprolidine CAS_486-12-4 NSC_5282443
1-((E)-3-Pyridin-2-yl-3-p-tolyl-allyl)-pyrrolidinium Myidyl Actidil 2-(3-Pyrrolidin-1-yl-1-p-tolyl-propenyl)-pyridine Allerfed 2-((E)-3-Pyrrolidin-1-yl-1-p-tolyl-propenyl)-pyridine Myfed Triprolidin (E)-2-(3-(pyrrolidin-1-yl)-1-p-tolylprop-1-enyl)pyridine Triphed Actifed BDBM50292411 Histafed CHEMBL855 Trilitron TRIPROLIDINE Corphed Triacin-C 2-(3-(pyrrolidin-1-yl)-1-p-tolylprop-1-enyl)pyridine Actahist

Scintillation Proximity Assay [3H]-Pyrilamine binding experiments are carried out in SPA (scintillation proximity assay) 96-well format. Membranes used in this assay are prepared from HEK-293 cells stably expressing recombinant H1 receptor (human). The incubation is initiated by the addition of a mixture of WGA PVT SPA beads (1 mg/well, Perkin Elmer (MA, USA) RPNQ0001) and 3 μg membranes to assay buffer (67 mM Tris; pH 7.6) containing 3.5 nM [3H]-Pyrilamine and varying concentrations of the test compound (10 point concentration response curves). Non-specific binding is determined in the presence of 10 μM Triprolidine. Samples are incubated for 4 hr. at room temperature (22� C.) and then read in a Microbeta Trilux.