Query String: ZILEUTON
- BDBM50320449 CHEMBL1164975 (-)-ZILEUTON
- CHEMBL1743359 ZILEUTON BDBM50418083
- N-(1-Benzo(b)thien-2-ylethyl)-N-hydroxyurea ZILEUTON N-[1-(benzo[b]thiophen-2-yl)ethyl]-N-hydroxyurea (+-)-1-(1-Benzo[b]thien-2-ylethyl)-1-hydroxyurea BDBM50000541 CHEMBL93 1-[1-(1-benzothien-2-yl)ethyl]-1-hydroxyurea Leutrol Zyflo (TN)
- Cell-Free Assay The enzyme activity of 5-LOX was determined fluorescence spectrophotometrically by oxidation of the substrate H2DCFDA to the highly fluorescent 2',7'-dichlorofluorescein product during 5-LOX┐s catalytic reaction . The reactions were initiated by the addition of AA as substrate and then monitored by excitation at 500 nm and emission at 520 nm utilizing a multiwall fluorometer. Fluorescence signals were recorded for 5 min with a kinetics mode program. Inhibition activities were measured as described previously. All inhibition values were tested at the concentration of 91 ÁM. Zileuton (reported IC50 = 0.9 ÁM) was used as positive control.