Affinity DataEC50: 0.00400nMAssay Description:Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated controlMore data for this Ligand-Target Pair
Affinity DataEC50: 0.00700nMAssay Description:In vitro agonist effect on estrogen receptor alpha transcriptional activation in MCF-7 cells at 10 pMMore data for this Ligand-Target Pair
Affinity DataEC50: 0.00700nMAssay Description:Displacement of [3H]17-beta-estradiol from human Estrogen receptor alphaMore data for this Ligand-Target Pair
Affinity DataEC50: 0.00840nMAssay Description:Agonist activity at human ERalpha in human MCF7 cells assessed as progesterone receptor endogenous genes activation after 24 hrs by qPCRMore data for this Ligand-Target Pair
Affinity DataEC50: 0.0100nMAssay Description:Effect on ERalpha in MCF7 cell line transfected with ER responsive luciferase reporterMore data for this Ligand-Target Pair
Affinity DataEC50: 0.0140nMAssay Description:Modulation of ER-alpha (unknown origin)More data for this Ligand-Target Pair
Affinity DataEC50: 0.0150nMAssay Description:Agonist activity at ER in human MCF7:WS8 cells assessed as increase in cell growth by measuring DNA level after 7 days by fluorescence analysisMore data for this Ligand-Target Pair
Affinity DataEC50: 0.0180nMAssay Description:Transcriptional potency (EC50) at Human estrogen receptor alphaMore data for this Ligand-Target Pair
Affinity DataEC50: 0.0190nMAssay Description:Transcriptional activation of estrogen receptor alpha in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-3...More data for this Ligand-Target Pair
Affinity DataIC50: 24nM EC50: 0.0200nMpH: 7.4 T: 2°CAssay Description:Radioligand binding assay was performed by using 96-well microtiterplates containing ER, 17beta-estradiol, and the test compound to be tested and SPA...More data for this Ligand-Target Pair
Affinity DataEC50: 0.0240nMAssay Description:Agonist activity at human ERalpha expressed in HEK293 cells by luciferase reporter gene assayMore data for this Ligand-Target Pair
Affinity DataEC50: 0.0280nMAssay Description:Estrogenic activity at ERalpha in human Ishikawa cells assessed as stimulation of alkaline phosphatase activity after 4 days by microplate scanning s...More data for this Ligand-Target Pair
Affinity DataEC50: 0.0290nMAssay Description:The relative efficacies of Formula I compounds as inhibitors of an enzyme activity (or other biological activity) can be established by determining t...More data for this Ligand-Target Pair
Ligand Info
Affinity DataEC50: 0.0300nMAssay Description:Estrogenic activity at ERalpha in human Ishikawa cells assessed as induction of alkaline phosphatase activity using p-nitrophenol as substrate treate...More data for this Ligand-Target Pair
Affinity DataEC50: 0.0360nMAssay Description:The relative efficacies of Formula I compounds as inhibitors of an enzyme activity (or other biological activity) can be established by determining t...More data for this Ligand-Target Pair
Ligand Info
Affinity DataEC50: 0.0370nMAssay Description:The relative efficacies of Formula I compounds as inhibitors of an enzyme activity (or other biological activity) can be established by determining t...More data for this Ligand-Target Pair
Ligand Info
Affinity DataEC50: 0.0380nMAssay Description:The relative efficacies of Formula I compounds as inhibitors of an enzyme activity (or other biological activity) can be established by determining t...More data for this Ligand-Target Pair
Ligand Info
Affinity DataEC50: 0.0400nMAssay Description:The relative efficacies of Formula I compounds as inhibitors of an enzyme activity (or other biological activity) can be established by determining t...More data for this Ligand-Target Pair
Ligand Info
Affinity DataEC50: 0.0460nMAssay Description:Agonist activity at ERalpha expressed in HEK cells co-expressing beta-lactamase after 18 hrs by beta-lactamase reporter gene assayMore data for this Ligand-Target Pair
Affinity DataEC50: 0.0460nMAssay Description:The relative efficacies of Formula I compounds as inhibitors of an enzyme activity (or other biological activity) can be established by determining t...More data for this Ligand-Target Pair
Ligand Info
Affinity DataEC50: 0.0470nMAssay Description:Estrogenic activity at estrogen receptor in human Ishikawa Var-1 cells assessed as stimulation of alkaline phosphatase activity measured by metabolis...More data for this Ligand-Target Pair
Affinity DataEC50: 0.0500nMAssay Description:Transcriptional activation of ERalpha receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assayMore data for this Ligand-Target Pair
Affinity DataEC50: 0.0590nMAssay Description:Transcriptional activation of estrogen receptor alpha in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-3...More data for this Ligand-Target Pair
Affinity DataIC50: 12nM EC50: 0.0600nMpH: 7.4 T: 2°CAssay Description:Radioligand binding assay was performed by using 96-well microtiterplates containing ER, 17beta-estradiol, and the test compound to be tested and SPA...More data for this Ligand-Target Pair
Affinity DataEC50: 0.0640nMAssay Description:Transcriptional potency (EC50) at Human estrogen receptor alphaMore data for this Ligand-Target Pair
Affinity DataEC50: 0.0700nMAssay Description:Induction of ERalpha degradation in tamoxifen-sensitive human MCF7:WS8 cells after 24 hrs by CellTag 700 staining based In-cell western assayMore data for this Ligand-Target Pair
Affinity DataEC50: 0.0700nMAssay Description:Agonist effect on transcriptional activation in T47D cells expressing estrogen receptor alphaMore data for this Ligand-Target Pair
Affinity DataEC50: 0.0800nMAssay Description:Concentration required to activate luciferase expression in MCF-7-2a cellsMore data for this Ligand-Target Pair
Affinity DataEC50: 0.0900nMAssay Description:Agonist activity at human full-length ERalpha receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional ac...More data for this Ligand-Target Pair
Affinity DataEC50: 0.0900nMAssay Description:Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalph...More data for this Ligand-Target Pair
Affinity DataEC50: 0.100nMAssay Description:The relative efficacies of Formula I compounds as inhibitors of an enzyme activity (or other biological activity) can be established by determining t...More data for this Ligand-Target Pair
Ligand Info
Affinity DataEC50: 0.100nMAssay Description:Induction of degradation of ERalpha in human MCF7 cells incubated for 4 hrs in presence of anti-ERalpha rabbit monoclonal antibodies measured after 4...More data for this Ligand-Target Pair
Affinity DataEC50: 0.100nMAssay Description:Agonist activity at ERalpha (unknown origin) expressed in human HepG2 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase rep...More data for this Ligand-Target Pair
Affinity DataEC50: 0.100nMAssay Description:Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated controlMore data for this Ligand-Target Pair
Affinity DataEC50: 0.100nMAssay Description:Activation of ERalpha in human MCF7/2a cells by luciferase reporter gene assay relative to untreated controlMore data for this Ligand-Target Pair
Affinity DataEC50: 0.100nMAssay Description:Agonist activity at ERalpha expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter ...More data for this Ligand-Target Pair
Affinity DataEC50: 0.100nMAssay Description:Induction of estrogen receptor-alpha degradation in human MCF7 cells after 4 hrs by in-cell western assayMore data for this Ligand-Target Pair
Affinity DataEC50: 0.107nMAssay Description:The relative efficacies of Formula I compounds as inhibitors of an enzyme activity (or other biological activity) can be established by determining t...More data for this Ligand-Target Pair
Ligand Info
Affinity DataEC50: 0.107nMAssay Description:Agonist activity at GAL4 DNA binding domain fused full-length chimeric estrogen receptor alpha (unknown origin) by FRET-based assayMore data for this Ligand-Target Pair
Affinity DataEC50: 0.110nMAssay Description:Agonist activity at recombinant human GAL4-fused ERalpha expressed in HEk293 cells by luciferase reporter gene assayMore data for this Ligand-Target Pair
Affinity DataEC50: 0.110nMAssay Description:Activity at human ERalpha expressed in HEC1 cells cotransfected with 2ERE-pS2-Luc assessed as relative transcriptional potency by luciferase reporter...More data for this Ligand-Target Pair
Affinity DataEC50: 0.110nMAssay Description:Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalph...More data for this Ligand-Target Pair
Affinity DataEC50: 0.110nMAssay Description:Activity at human ERalpha expressed in HEC1 cells cotransfected with 2ERE-pS2-Luc assessed as relative transcriptional potency by luciferase reporter...More data for this Ligand-Target Pair
Affinity DataEC50: 0.120nMAssay Description:Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated controlMore data for this Ligand-Target Pair
Affinity DataEC50: 0.123nMAssay Description:Agonist activity at human ERalpha expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assayMore data for this Ligand-Target Pair
Affinity DataEC50: 0.130nMAssay Description:Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activit...More data for this Ligand-Target Pair
Affinity DataEC50: 0.140nMAssay Description:Agonist activity at human ERalpha expressed in HepG2 cells after 24 hrs by luciferase reporter gene assayMore data for this Ligand-Target Pair
Affinity DataEC50: 0.150nMAssay Description:Agonist activity at ER in human MCF7:WS8 cells assessed as increase in cell growth by measuring DNA level after 7 days by fluorescence analysisMore data for this Ligand-Target Pair
Affinity DataEC50: 0.150nMAssay Description:Agonist activity at human ERalpha expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assayMore data for this Ligand-Target Pair
Affinity DataEC50: 0.160nMAssay Description:Agonist activity at human ERalpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene t...More data for this Ligand-Target Pair