TargetProtein arginine N-methyltransferase 8(Homo sapiens (Human))
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 1.10nMAssay Description:Inhibition of PRMT8 (unknown origin) incubated for 15 mins followed by substrate addition measured after 60 mins by AlphaLisa methodMore data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 1(Rattus norvegicus)
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 1.10nMAssay Description:Inhibition of rat His-tagged PRMT1 (11 to 353 residues) expressed in Escherichia coli BL21(DE3) incubated for 15 mins followed by substrate addition ...More data for this Ligand-Target Pair
Affinity DataIC50: 1.20nMAssay Description:The assays were all performed in a buffer consisting of 20 mM Bicine (pH=7.6), 1 mM TCEP, 0.005% Bovine Skin Gelatin, and 0.002% Tween20, prepared on...More data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 8(Homo sapiens (Human))
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 2nMAssay Description:Inhibition of N-terminal GST tagged full-length human PRMT8 expressed in Escherichia coli (BL21(DE3) pre-incubated for 30 mins before addition of a [...More data for this Ligand-Target Pair
Affinity DataIC50: 2.64nMAssay Description:For the detection of SMYD2 cellular methylation activity an In Cell Western (ICW) assay was established. This assay allows rapid processing of multip...More data for this Ligand-Target Pair
Affinity DataIC50: 2.80nMAssay Description:Inhibition of SMYD2 (unknown origin) using biotinylated GSRAHSSHLKSKKGQSTSRH as substrate assessed as incorporation of tritium labeled methyl group f...More data for this Ligand-Target Pair
Affinity DataIC50: 2.90nMAssay Description:Inhibition of human SMYD2 overexpressing human KYSE-150 cells assessed as SMYD2 assessed as reduction in BTF3 monomethylation by Western blot analysi...More data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Inhibition of SMYD2 (unknown origin) using 3H-SAM as substrate incubated for 1 hr by filter binding methodMore data for this Ligand-Target Pair
Affinity DataIC50: 3.30nMAssay Description:The assays were all performed in a buffer consisting of 20 mM Bicine (pH=7.6), 1 mM TCEP, 0.005% Bovine Skin Gelatin, and 0.002% Tween20, prepared on...More data for this Ligand-Target Pair
Affinity DataIC50: 3.90nMAssay Description:The assays were all performed in a buffer consisting of 20 mM Bicine (pH=7.6), 1 mM TCEP, 0.005% Bovine Skin Gelatin, and 0.002% Tween20, prepared on...More data for this Ligand-Target Pair
Affinity DataIC50: 3.90nMAssay Description:Inhibition of FLAG and C-terminally Avi-tagged full length SMYD2 (unknown origin) expressed in Sf9 cells using SAM and histone H3 (1 to 29 residues) ...More data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 8(Homo sapiens (Human))
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 4nMAssay Description:Inhibition of PRMT8 (unknown origin) by scintillation proximity assayMore data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 4nMAssay Description:This is a review article. Please point to the original journal.More data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 8(Homo sapiens (Human))
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 4nMAssay Description:Inhibition of PRMT8 (unknown origin) using biotinylated histone H4 (1 to 24 residues) as substrate in presence of [3H]SAM by scintillation proximity ...More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 4nMAssay Description:The SARS 3CLpro FRET assay measures the protease catalyzed cleavage of TAMRA- SITSAVLQSGFRKMK-(DABCYL)-OH to TAMRA - SITSAVLQ and SGFRKMK- (DABCYL)-O...More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 4nMAssay Description:Please point to the patents.More data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 8(Homo sapiens (Human))
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 4nMAssay Description:Inhibition of human PRMT8 assessed as inhibition of methylation activity using biotin-labeled peptide as substrate and [3H]-SAM by scintillation prox...More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 4nMAssay Description:The assays are from references cited in this article.More data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 8(Homo sapiens (Human))
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataKi: 1.30nM IC50: 5nMAssay Description:In brief, the tritiated S-adenosyl-L-methionine (3HSAM, PerkinElmer Life Sciences) was used as the donor of methyl group. The (3H) methylated biotin ...More data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 1(Rattus norvegicus)
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 5.30nMAssay Description:Inhibition of rat His-tagged PRMT1 (11 to 353 residues) expressed in Escherichia coli BL21(DE3) using biotinylated H4 peptide (1 to 21 residues) as s...More data for this Ligand-Target Pair
Affinity DataIC50: 5.55nMpH: 9.0Assay Description:SMYD2 inhibitory activities of the compounds described in the present invention were quantified using a scintillation proximity assay (SPA) which mea...More data for this Ligand-Target Pair
Affinity DataIC50: 5.60nMAssay Description:Inhibition of SMYD2 (unknown origin) using biotinylated GSRAHSSHLKSKKGQSTSRH as substrate assessed as incorporation of tritium labeled methyl group f...More data for this Ligand-Target Pair
Affinity DataIC50: 6nMAssay Description:Inhibition of recombinant human SMYD2 (1 to 433 residues) expressed in baculovirus infected Sf9 insect cells using biotinylated-p53 peptide (361 to 3...More data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 1(Rattus norvegicus)
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 6nMAssay Description:Inhibition of rat His-tagged PRMT1 (11 to 353 residues) expressed in Escherichia coli BL21(DE3) using biotinylated H4 peptide (1 to 21 residues) as s...More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 6.60nMAssay Description:Please point to the patents.More data for this Ligand-Target Pair
Affinity DataIC50: 6.65nMpH: 9.0Assay Description:SMYD2 inhibitory activities of the compounds described in the present invention were quantified using a scintillation proximity assay (SPA) which mea...More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 6.80nMAssay Description:Please point to the patents.More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 7nMAssay Description:Please point to the patents.More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 7nMAssay Description:Please point to the patents.More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 7nMAssay Description:Please point to the patents.More data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 8(Homo sapiens (Human))
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 7nMAssay Description:Inhibition of N-terminal GST tagged full-length human PRMT8 expressed in Escherichia coli (BL21(DE3) pre-incubated for 30 mins before addition of a [...More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 7nMAssay Description:The assays are from references cited in this article.More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 7nMAssay Description:This is a review article. Please point to the original journal.More data for this Ligand-Target Pair
Affinity DataIC50: 7.80nMAssay Description:The assays were all performed in a buffer consisting of 20 mM Bicine (pH=7.6), 1 mM TCEP, 0.005% Bovine Skin Gelatin, and 0.002% Tween20, prepared on...More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 8nMAssay Description:SARS-CoV-2 3CLpro expression and purification is based on a published procedure and our modified protocol is found in the supplementary file. A highl...More data for this Ligand-Target Pair
Affinity DataIC50: 8nMAssay Description:Inhibition of recombinant human SMYD2 (1 to 433 residues) expressed in baculovirus infected Sf9 insect cells using biotinylated-p53 peptide (361 to 3...More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 9nMAssay Description:SARS-CoV-2 3CLpro expression and purification is based on a published procedure and our modified protocol is found in the supplementary file. A highl...More data for this Ligand-Target Pair
Affinity DataIC50: 9.09nMpH: 9.0Assay Description:SMYD2 inhibitory activities of the compounds described in the present invention were quantified using a scintillation proximity assay (SPA) which mea...More data for this Ligand-Target Pair
Affinity DataIC50: 9.98nMpH: 9.0Assay Description:SMYD2 inhibitory activities of the compounds described in the present invention were quantified using a scintillation proximity assay (SPA) which mea...More data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 8(Homo sapiens (Human))
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 10nMAssay Description:Inhibition of N-terminal GST tagged full-length human PRMT8 expressed in Escherichia coli (BL21(DE3) pre-incubated for 30 mins before addition of a [...More data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 8(Homo sapiens (Human))
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 10nMAssay Description:Inhibition of N-terminal GST tagged full-length human PRMT8 expressed in Escherichia coli (BL21(DE3) pre-incubated for 30 mins before addition of a [...More data for this Ligand-Target Pair
TargetProtein arginine N-methyltransferase 8(Homo sapiens (Human))
Zhejiang Sci-Tech University
Curated by ChEMBL
Zhejiang Sci-Tech University
Curated by ChEMBL
Affinity DataIC50: 10nMAssay Description:Inhibition of N-terminal GST tagged full-length human PRMT8 expressed in Escherichia coli (BL21(DE3) pre-incubated for 30 mins before addition of a [...More data for this Ligand-Target Pair
Affinity DataIC50: 11nMAssay Description:The assays were all performed in a buffer consisting of 20 mM Bicine (pH=7.6), 1 mM TCEP, 0.005% Bovine Skin Gelatin, and 0.002% Tween20, prepared on...More data for this Ligand-Target Pair
Affinity DataIC50: 12nMAssay Description:Inhibition of SMYD2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 12.5nMpH: 9.0Assay Description:SMYD2 inhibitory activities of the compounds described in the present invention were quantified using a scintillation proximity assay (SPA) which mea...More data for this Ligand-Target Pair
Affinity DataIC50: 13nMAssay Description:The assays were all performed in a buffer consisting of 20 mM Bicine (pH=7.6), 1 mM TCEP, 0.005% Bovine Skin Gelatin, and 0.002% Tween20, prepared on...More data for this Ligand-Target Pair
Affinity DataIC50: 13nMpH: 9.0Assay Description:SMYD2 inhibitory activities of the compounds described in the present invention were quantified using a scintillation proximity assay (SPA) which mea...More data for this Ligand-Target Pair
Affinity DataIC50: 13.2nMAssay Description:For the detection of SMYD2 cellular methylation activity an In Cell Western (ICW) assay was established. This assay allows rapid processing of multip...More data for this Ligand-Target Pair
Affinity DataIC50: 13.5nMpH: 9.0Assay Description:SMYD2 inhibitory activities of the compounds described in the present invention were quantified using a scintillation proximity assay (SPA) which mea...More data for this Ligand-Target Pair
TargetReplicase polyprotein 1ab(Human SARS coronavirus (SARS-CoV) (Severe acute re...)
Michigan State University
Michigan State University
Affinity DataIC50: 14nMAssay Description:This is a review article.More data for this Ligand-Target Pair