Found 50 Enz. Inhib. hit(s) for PDB: 1UWJ TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
3D Structure (crystal)TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKi: 38nM ΔG°: -44.1kJ/molepH: 7.0 T: 2°CAssay Description:BRAF kinase activity was quantified using an ELISA-based MEK phosphorylation assay. IC50 values were derived from the sigmoidal dose-response curve f...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 4.40nMAssay Description:Inhibition of BRAF V600E mutant in human A375 cells assessed as reduction in ERK phosphorylationMore data for this Ligand-Target Pair
Affinity DataIC50: 11nMAssay Description:To measure compound inhibitor effects on the ability of Raf enzymes to phosphorylate the Mek substrate, a capture ELISA utilizing streptavidin-coated...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 12nMAssay Description:Inhibition of B-Raf (unknown origin) using poly (Glu, Tyr) 4:1 substrate incubated for 30 mins by HTRF assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 12nMAssay Description:Inhibition of purified B-Raf (unknown origin) after 30 mins by homogeneous time-resolved fluorescence assayMore data for this Ligand-Target Pair
Affinity DataIC50: 12.7nMpH: 7.5Assay Description:Inhibitor Concentration at 50% enzyme inhibition (IC50) values were calculated by quantifying the end-point ADP production from each kinase reaction ...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 15nMAssay Description:Inhibition of B-RAF kinaseMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of recombinant BRAF (409 to 765 residues) (unknown origin) using MEK1 as substrate after 25 mins in presence of gamma-[33P]ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 22nMpH: 8.2 T: 2°CAssay Description:The biochemical activity of compound was determined by incubation with Raf kinases and substrate MEK-1 in the presence of 1-10 uM ATP/ [gamma-32P] AT...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of B-Raf kinaseMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of wild type B-Raf assessed as inhibition of MEK1 phosphorylation using [gamma-33P]ATP by beta countingMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of B-Raf in presence of 5 uM ATPMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of recombinant BRAF (409 to 765 residues) (unknown origin) expressed in baculovirus expression system using MEk1 as substrate after 25 min...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 22nMAssay Description:Inhibition of B-Raf (unknown origin) using MEK1 as substrate measured after 2 hrs by ELISAMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 23nMAssay Description:Inhibition of wild-type BRAF (unknown origin) using human His6-tagged MEK1 K97R mutant as substrate pretreated for 20 mins followed by [33P]-ATP addi...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 24nMAssay Description:Inhibition of B-Raf V600E mutant (unknown origin) using MEK1 as substrate measured after 2 hrs by ELISAMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 26nMAssay Description:Inhibition of human GST-tagged full length B-Raf V600E mutant (unknown origin) using His6-tagged full-length human MEK1 (K97R) substrate and [33P]-ga...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 38nMAssay Description:Inhibition of recombinant BRAF (409 to 765 residues) V599E mutant (unknown origin) expressed in baculovirus expression system using MEk1 as substrate...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 38nMAssay Description:Inhibition of BRAF V600E mutant (unknown origin) measured after 40 mins by scintillation countingMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 38nMAssay Description:Inhibition of BRAF (unknown origin) incubated for 15 mins by fluorescence based assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf [410-766,V600E](Homo sapiens (Human))
Bayer Pharmaceuticals
Bayer Pharmaceuticals
Affinity DataIC50: 38nMpH: 8.2 T: 2°CAssay Description:The biochemical activity of compound was determined by incubation with Raf kinases and substrate MEK-1 in the presence of 1-10 uM ATP/ [gamma-32P] AT...More data for this Ligand-Target Pair
Affinity DataIC50: 42nMpH: 7.5 T: 2°CAssay Description:Compounds were prepared in DMSO at 10mmol/L and serially diluted in a 96-well plate as the source plate. The intermediate plate consists of 4µL...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 43nMAssay Description:Inhibition of B-Raf (unknown origin) after 1 hr by ADP-Glo assayMore data for this Ligand-Target Pair
Affinity DataIC50: 43nMpH: 7.2 T: 2°CAssay Description:The biological activities (IC50s) of the compounds were determined against the B-RAF (V600E) mutant enzyme in vitro. GST-MEK1, B-RAF (V600E), and inh...More data for this Ligand-Target Pair
Affinity DataIC50: 43nMAssay Description:BRAF kinase activity was quantified using a DELFIA-based MEK1 phosphorylation assay. IC50 values were derived from the sigmoidal dose-response curves...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 43nMAssay Description:Inhibition of BRAF V600E mutantMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 46nMAssay Description:Inhibitory activity against human B-RAFMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 52nMAssay Description:Inhibition of BRAF V600E mutant (unknown origin) by Z'-Lyte assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 76.2nMAssay Description:Inhibition of human recombinant full-length GST-tagged B-Raf by radiometric kinase assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 114nMpH: 7.5 T: 2°CAssay Description:Compounds were prepared in DMSO at 10mmol/L and serially diluted in a 96-well plate as the source plate. The intermediate plate consists of 4µL...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 120nMAssay Description:Binding affinity to B-RAF kinase (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 120nMAssay Description:Competitive inhibition of recombinant B-Raf (unknown origin) expressed in HEK293 cells by FRET analysis in presence of ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 130nMAssay Description:Inhibition of mouse full length GST-tagged BRAF V600E mutant using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 136nMAssay Description:Competitive binding affinity to B-RAF kinase (unknown origin) after 60 to 120 mins by FRET analysisMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 310nMAssay Description:Inhibition of BRAF (unknown origin) incubated for 10 mins using FAM-labeled peptide and ATP by mobility shift assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 310nMAssay Description:Inhibition of BRAF (unknown origin) using FAM-labeled peptide as substrate after 1 hr by Lanthascreen assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 6.10E+3nMAssay Description:Growth inhibition in human melanoma cells WM266.4 expressing mutant B-RAF with SRBMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 6.60E+3nMAssay Description:Inhibition of BRAF V600E mutant-dependent MEK phosphorylation in A375 cellsMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataIC50: 7.30E+3nMAssay Description:Inhibition of B-Raf in the presence of 100uM ATPMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 540nMAssay Description:Kinase inhibitors are a new class of therapeutics with a propensity to inhibit multiple targets. The biological consequences of multi-kinase activity...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 284nMAssay Description:Binding affinity to BRAF V600E mutantMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 260nMAssay Description:Kinase inhibitors are a new class of therapeutics with a propensity to inhibit multiple targets. The biological consequences of multi-kinase activity...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 540nMAssay Description:Binding constant for BRAF kinase domainMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataEC50: 3nMAssay Description:Inhibition of B-raf by cellular assayMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 540nMAssay Description:Binding constant for BRAF kinase domainMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 260nMAssay Description:Binding constant for BRAF kinase domainMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 260nMAssay Description:Binding constant for BRAF(V600E) kinase domainMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf(Homo sapiens (Human))
University Of California San Francisco
Curated by ChEMBL
University Of California San Francisco
Curated by ChEMBL
Affinity DataKd: 540nMAssay Description:Binding affinity to BRAFMore data for this Ligand-Target Pair
