Found 56 Enz. Inhib. hit(s) for PDB: 3EYG 
Affinity DataKi: 0.680nMAssay Description:Inhibition of human recombinant GST-tagged JAK1 using 5-FAM-KKSRGDYMTMQIG as substrate by peptide mobility shift assayMore data for this Ligand-Target Pair
3D Structure (crystal)Affinity DataKi: 0.700nMAssay Description:Inhibition of recombinant JAK1 (unknown origin) using Val-Ala-Leu-Val-Asp-Gly-Tyr-Phe-Arg-Leu-Thr-Thr as substrate after 30 mins in presence of ATPMore data for this Ligand-Target Pair
Affinity DataKi: 0.700nMAssay Description:Inhibition of human GST-fused JAK1 catalytic domain expressed in baculovirus-infected Sf9 cells using polyglutamic acid-tyrosine as substrate after 3...More data for this Ligand-Target Pair
Affinity DataIC50: 0.496nMAssay Description:A commercial assay by Reaction Biology Corp.More data for this Ligand-Target Pair
Affinity DataIC50: 0.5nMAssay Description:Inhibition of human JAK1 using poly[Glu:Tyr] (4:1) as substrate in presence of 10 uM [gamma33P]ATP by radiometric methodMore data for this Ligand-Target Pair
Affinity DataIC50: 0.800nMAssay Description:Inhibition of human recombinant JAK1More data for this Ligand-Target Pair
Affinity DataIC50: 1.30nMAssay Description:Inhibition of JAK1 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 1.30nMAssay Description:Inhibition of GST-tagged recombinant human JAK1 catalytic domain expressed in baculovirus expression system using LCB-EQEDEPEGDYFEWLW-NH2 as substrat...More data for this Ligand-Target Pair
Affinity DataIC50: 1.40nMAssay Description:Inhibition of JAK1 (unknown origin) preincubated for 20 mins followed by [33P]-ATP addition measured after 2 hrs by filter-binding methodMore data for this Ligand-Target Pair
Affinity DataIC50: 1.42nMAssay Description:Reagents used in JAK inhibition assay: Base Reaction buffer; 20 mM Hepes (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.02% Brij35, 0.02 mg/ml BSA, 0.1 mM Na3VO...More data for this Ligand-Target Pair
Affinity DataIC50: 1.60nMAssay Description:Inhibition of recombinant human GST-tagged JAK1 catalytic domain (866 to 1154 residues) expressed in baculovirus expression system using TK-substrate...More data for this Ligand-Target Pair
Affinity DataIC50: 1.60nMAssay Description:Inhibition of JAK1 (unknown origin)-mediated phosphorylation of Biotin-KAIETDKEYYTVKD incubated for 10 mins prior to substrate addition measured afte...More data for this Ligand-Target Pair
Affinity DataIC50: 1.60nMAssay Description:Inhibition of JAK1 (unknown origin) using [33gammaP]ATP and Biotin-KAIETDKEYYTVKD as substrate incubated for 10 mins prior to substrate addition meas...More data for this Ligand-Target Pair
Affinity DataIC50: 2nMAssay Description:Inhibition of JAK1 (unknown origin)-mediated phosphorylation of Biotin-KAIETDKEYYTVKD incubated for 10 mins prior to substrate addition measured afte...More data for this Ligand-Target Pair
Affinity DataIC50: 2nMAssay Description:Inhibition of recombinant human JAK1 (866-end residues) in presence of ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 2nMAssay Description:Inhibition of JAK1 (unknown origin) using Biotin-KAIETDKEYYTVKD as substrate and [33Pgamma]ATP incubated for 10 mins prior to substrate addition meas...More data for this Ligand-Target Pair
Affinity DataIC50: 2.60nMpH: 7.4 T: 2°CAssay Description:Test compounds were solubilized in dimethyl sulfoxide (DMSO) to a stock concentration of 30 mM. Compounds were diluted in DMSO to create an 11-point ...More data for this Ligand-Target Pair
Affinity DataIC50: 3nMAssay Description:Inhibition of human recombinant N-terminal GST-tagged JAK1 expressed in Sf9 cells using Biotin-LC-EQEDEPEGDYFEWLE as substrate after 90 mins by TR-FR...More data for this Ligand-Target Pair
Affinity DataIC50: 3.10nMAssay Description:Inhibition of recombinant GST-tagged human JAK1 (852 to 1142 residues) using IRS-1 as substrate in presence of 40 uM ATP by mobility shift assayMore data for this Ligand-Target Pair
Affinity DataIC50: 3.20nMAssay Description:Inhibition of recombinant GST-fused JAK1 kinase domain (852 to 1142) (unknown origin) using 5-FAM-KKSRGDYMTMQIG as substrate assessed as inhibition o...More data for this Ligand-Target Pair
Affinity DataIC50: 3.30nMAssay Description:Inhibition of JAK1 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 3.70nMAssay Description:Inhibition of human JAK1 kinase domain using biotin-Lyn-substrate-2 as substrate and ATP incubated for 1 hr by ELISA methodMore data for this Ligand-Target Pair
Affinity DataIC50: 3.80nMAssay Description:Inhibition of JAK1 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 4nMpH: 7.5Assay Description:A kinase selectivity panel which measures substrate peptide phosphorylation was set-up for recombinant human Jak1 (aa 866-1154), Jak2 (aa808-1132), J...More data for this Ligand-Target Pair
Affinity DataIC50: 4.10nMAssay Description:Inhibition of JAK1 by solution phase kinase assayMore data for this Ligand-Target Pair
Affinity DataIC50: 6.10nMAssay Description:Inhibition of GST-tagged Jak1 expressed in insect cells using 70 uM ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 15nMAssay Description:Inhibition of recombinant human JAK1 using 5'FAM-KKSRGDYMTMQID as substrate in presence of 1 mM ATP by mobility shift assayMore data for this Ligand-Target Pair
Affinity DataIC50: 15nMAssay Description:Inhibition of JAK1 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 15nMAssay Description:Inhibition of JAK1 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 15nMpH: 7.4 T: 2°CAssay Description:Test compounds were solubilized in dimethyl sulfoxide (DMSO) to a stock concentration of 30 mM. Compounds were diluted in DMSO to create an 11-point ...More data for this Ligand-Target Pair
Affinity DataIC50: 26nMAssay Description:Inhibition of TEL-fused JAK1 expressed in Ba/F3 cells assessed as inhibition of STAT5 phosphorylation after 60 mins by AlphaScreen assayMore data for this Ligand-Target Pair
Affinity DataIC50: 28nMAssay Description:Inhibition of JAK3/1 in human T cells expressing CD3 assessed as inhibition of IL2-stimulated STAT5a phosphorylationMore data for this Ligand-Target Pair
Affinity DataIC50: 30nMAssay Description:Inhibition of JAK1/3 in human PBMC expressing CD3 assessed as inhibition of IL2-induced STAT5a phosphorylationMore data for this Ligand-Target Pair
TargetNon-receptor tyrosine-protein kinase TYK2/Tyrosine-protein kinase JAK1(Homo sapiens (Human))
Pfizer
Curated by ChEMBL
Pfizer
Curated by ChEMBL
Affinity DataIC50: 35nMAssay Description:Inhibition of JAK1/TYK2 in human whole blood assessed as reduction in IFNalpha induced STAT3 phosphorylation preincubated for 45 mins followed by IFN...More data for this Ligand-Target Pair
TargetNon-receptor tyrosine-protein kinase TYK2/Tyrosine-protein kinase JAK1(Homo sapiens (Human))
Pfizer
Curated by ChEMBL
Pfizer
Curated by ChEMBL
Affinity DataIC50: 35nMAssay Description:Inhibition of JAK1/TYK2 in human whole blood assessed as inhibition of IFN-alpha-induced STAT-3 phosphorylation preincubated for 45 mins followed by ...More data for this Ligand-Target Pair
Affinity DataIC50: 56nMAssay Description:Inhibition of JAK1/JAK3 in human whole blood assessed as inhibition of IL-15-induced STAT-5 phosphorylation preincubated for 45 mins followed by IL-1...More data for this Ligand-Target Pair
Affinity DataIC50: 74nMAssay Description:Inhibition of JAK1 in human whole blood assessed as inhibition of IL-6-induced pSTAT1More data for this Ligand-Target Pair
TargetNon-receptor tyrosine-protein kinase TYK2/Tyrosine-protein kinase JAK1/Tyrosine-protein kinase JAK2(Homo sapiens (Human))
Pfizer
Curated by ChEMBL
Pfizer
Curated by ChEMBL
Affinity DataIC50: 75nMAssay Description:Inhibition of JAK1/JAK2/TYK2 in human whole blood assessed as inhibition of IL-6-induced STAT-1 phosphorylation preincubated for 45 mins followed by ...More data for this Ligand-Target Pair
Affinity DataIC50: 95nMAssay Description:Inhibition of JAK1 in human PBMC cells assessed as inhibition of IL-6-induced MCP1 secretionMore data for this Ligand-Target Pair
Affinity DataIC50: 110nMAssay Description:Inhibition of GST-tagged human JAK1 catalytic domain expressed in Sf9 cells by ELISAMore data for this Ligand-Target Pair
Affinity DataIC50: 124nMAssay Description:Displacement of [35S]-MK499 from human ERG expressed in HEK293 cell membranesMore data for this Ligand-Target Pair
Affinity DataIC50: 170nMAssay Description:Inhibition of JAK1/2 in human PBMC expressing CD14 assessed as inhibition of IFNgamma-induced STAT1 phosphorylationMore data for this Ligand-Target Pair
Affinity DataIC50: 170nMAssay Description:Inhibition of JAK2/1 in human T cells expressing CD3 assessed as inhibition of IFNgamma-stimulated STAT1 phosphorylationMore data for this Ligand-Target Pair
Affinity DataKd: >1.00E+4nMAssay Description:Binding affinity to JAK1More data for this Ligand-Target Pair
Affinity DataEC50: 53nMAssay Description:Inhibition of JAK1 (unknown origin) assessed as inhibition of STAT3 phosphorylation by cell-based assayMore data for this Ligand-Target Pair
Affinity DataEC50: 53nMAssay Description:Inhibition of JAK1 in human TF1 cells assessed as inhibition of IL2-induced STAT3 phosphorylationMore data for this Ligand-Target Pair
Affinity DataKd: >1.00E+4nMAssay Description:Binding constant for JAK1(Kin.Dom.1/JH2 - pseudokinase) kinase domainMore data for this Ligand-Target Pair
