Found 10 Enz. Inhib. hit(s) with all data for entry = 7929 Target26S proteasome non-ATPase regulatory subunit 14(Homo sapiens (Human))
California Institute Of Technology
California Institute Of Technology
Affinity DataIC50: 340nMpH: 7.5 T: 2°CAssay Description:Fluorescence polarization assays were performed in low-volume 384-well solid black plates (Molecular Devices) in quadruplicate. The assays were perfo...More data for this Ligand-Target Pair
TargetBRISC and BRCA1-A complex member 1/BRISC and BRCA1-A complex member 2/BRISC complex subunit Abraxas 2/Lys-63-specific deubiquitinase BRCC36(Homo sapiens (Human))
California Institute Of Technology
California Institute Of Technology
Affinity DataIC50: 2.30E+3nMAssay Description:BRCC36 activity was measured using 1 nM purified BRISC complex and 80 nM di-ubiquitin substrate DiUbK63TAMRA (UF-310, Boston Biochem). TAMRA fluoresc...More data for this Ligand-Target Pair
Target26S proteasome non-ATPase regulatory subunit 14(Homo sapiens (Human))
California Institute Of Technology
California Institute Of Technology
Affinity DataIC50: 2.40E+3nMpH: 7.5 T: 2°CAssay Description:Fluorescence polarization assays were performed in low-volume 384-well solid black plates (Molecular Devices) in quadruplicate. The assays were perfo...More data for this Ligand-Target Pair
Affinity DataIC50: 4.50E+3nMAssay Description:AMSH activity was measured using 10 nM purified AMSH (E-548B, Boston Biochem) and 80 nM di-ubiquitin substrate DiUbK63TAMRA (UF-310, Boston Biochem)....More data for this Ligand-Target Pair
Affinity DataIC50: 3.00E+4nMpH: 7.5Assay Description:Csn5 activity was measured using purified CSN complex and the fluorescent substrate OGNedd8–SCFSkp2, in which a unique cysteine engineered into ...More data for this Ligand-Target Pair
Affinity DataIC50: 4.28E+4nMpH: 7.0 T: 2°CAssay Description:Assays were carried out in 100 mM sodium phosphate, pH 7.0 buffer using 96-well Clear UV Plate (Corning UV Transparent Microplates; catalog #3635). A...More data for this Ligand-Target Pair
Affinity DataIC50: >5.00E+4nMpH: 7.5 T: 2°CAssay Description:The assay was carried out in white NUNC 96-well plates as previously described [Puerta et al., J. Biol. Inorg. Chem., 11:131-138]. Each well containe...More data for this Ligand-Target Pair
Affinity DataIC50: >5.00E+4nMpH: 7.5 T: 2°CAssay Description:The assay was carried out in white NUNC 96-well plates as previously described [Puerta et al., J. Biol. Inorg. Chem., 11:131-138]. Each well containe...More data for this Ligand-Target Pair
Affinity DataIC50: >5.00E+4nMpH: 8.0 T: 2°CAssay Description:Assays were run in 50 mM HEPES (pH 8.0). A BioTek Synergy H4 microplate sample processor was used. The compounds were incubated with protein (final c...More data for this Ligand-Target Pair
Affinity DataIC50: >2.00E+5nMpH: 8.0 T: 2°CAssay Description:The enzyme was diluted with 25 mM Tris-Cl, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA, pH 8.0 buffer and its activity was measured by using S...More data for this Ligand-Target Pair
