Cell Reactant:

Syringe Reactant:

Meas. Tech.:

ITC

Entry Date.:

12/13/00

ΔG°:

-79.38±1.47 (kJ/mole)

pH:

7.5±n/a

Temperature:

283.15±n/a (K)

ΔH° :

-106.68±5.334 (kJ/mole)

Corrected for ΔH_ioniz:

yes

Protons Released:

-0.35

ΔC_p :

-2.14±0.084 (kJ/mole)

ΔS° :

-0.089±0.22 (kJ/mole-K)

Citation

 Perozzo, R; Jelesarov, I; Bosshard, HR; Folkers, G; Scapozza, L Compulsory order of substrate binding to herpes simplex virus type 1 thymidine kinase. A calorimetric study. J Biol Chem 275:16139-45 (2000) [PubMed]  Article

More Info.:

Get all data from this article,  ITC RUN data,  Solution Info,  Instrument Info

Source:

Expressed as glutathione S-transferase fusion protein in E. coli.

Prep. Method:

Purified by glutathione affinity chromatography.

Name:

Thymidine kinase

Synonyms:

KITH_HHV11 | TK

Type:

Enzyme

Mol. Mass.:

40978.57

Organism:

Human herpesvirus 1

Description:

P03176

Residue:

376

Sequence:

MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQEATEVRPEQKMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN

Blast this sequence in BindingDB or PDB

  
Blast E-value cutoff:

Source:

Sigma

Name:

BDBM3

Synonyms:

thymidine/ATP (1:1 mixture)

Type:

n/a

Emp. Form.:

n/a

Mol. Mass.:

n/a

SMILES:

n/a

Structure: