Cell Reactant:
Thymidine Kinase
Syringe Reactant:
BDBM3
Meas. Tech.:
ITC
Entry Date.:
12/13/00
ΔG°:
-79.38±1.47 (kJ/mole)
pH:
7.5±n/a
Temperature:
283.15±n/a (K)
ΔH° :
-106.68±5.334 (kJ/mole)
Corrected for ΔHioniz:
yes
Protons Released:
-0.35
ΔCp :
-2.14±0.084 (kJ/mole)
ΔS° :
-0.089±0.22 (kJ/mole-K)
Citation
 Perozzo, RJelesarov, IBosshard, HRFolkers, GScapozza, L Compulsory order of substrate binding to herpes simplex virus type 1 thymidine kinase. A calorimetric study. J Biol Chem 275:16139-45 (2000) [PubMed]  Article
Cell React
Source:
Expressed as glutathione S-transferase fusion protein in E. coli.
Prep. Method:
Purified by glutathione affinity chromatography.
Name:
Thymidine kinase
Synonyms:
n/a
Type:
Enzyme
Mol. Mass.:
40978.57
Organism:
Human herpesvirus 1
Description:
P03176
Residue:
376
Sequence:
MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQEATEVRPEQKMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN
  
Syringe React
Source:
Sigma
Name:
BDBM3
Synonyms:
thymidine/ATP (1:1 mixture)
Type:
n/a
Emp. Form.:
n/a
Mol. Mass.:
n/a
SMILES:
n/a
Structure: