Reaction Details  Report a problem with these data
Cell Reactant:
HyHEL-5 Mutant(E50Q)
Syringe Reactant:
Lysozyme (bobwhite quail)
Meas. Tech.:
ITC and sedimentation equilibrium
Entry Date.:
12/13/00
ΔG°:
-28.14±5.04 (kJ/mole)
pH:
8±n/a
Temperature:
298.15±n/a (K)
Corrected for ΔHioniz:
not known
Comments:
affinity was too low to measured through ITC
Citation
 Wibbenmeyer, JA; Schuck, P; Smith-Gill, SJ; Willson, RC Salt links dominate affinity of antibody HyHEL-5 for lysozyme through enthalpic contributions. J Biol Chem 274:26838-42 (1999) [PubMed]  Article
Cell React
Source:
Site-directed mutagenesis of recombinant HyHEL-5 Fab
Purity:
95%
Prep. Method:
Refolded protein was purified by Q Sepharose and nickel-charged chelating Sepharose Fast Flow Chromatography
Name:
HyHEL-5 Mutant(E50Q)
Synonyms:
HyHEL-5 Fab mutant (E50Q)
Type:
Antibody
Mol. Mass.:
23011.87
Organism:
Mus musculus (mouse)
Description:
mutant of murine monoclonal antibody HyHEL-5 to hen egg lysozyme
Residue:
215
Sequence:
EVQLQQSGAELMKPGASVKISCKASGYTFSDYWIEWVKQRPGHGLEWIGQILPGSGSTNYHERFKGKATFTADTSSSTAYMQLNSLTSEDSGVYYCLHGNYDFDGWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKILD
  
Syringe React
Source:
Prepared from eggs purchased from Stevenson's Game Bird Farm (Riverside, TX)
Name:
Lysozyme (bobwhite quail)
Synonyms:
LYSC_COLVI | LYZ | bobwhite quail lysozyme(BQL)
Type:
Enzyme
Mol. Mass.:
14279.53
Organism:
Colinus virginianus
Description:
hydrolase
Residue:
129
Sequence:
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNSQATNRNTDGSTDYGVLQINSRWWCNDGKTPGSRNLCNIPCSALLSSDITATVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL