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Cell Reactant:Streptavadin(W79F)
Syringe Reactant:BDBM12
Meas. Tech.:Isothermal Titration Calorimetry
Entry Date:04/30/08
 
Δ G ° :n/a
pH:n/a
Log10Kb:n/a
Temperature:298.45±n/a (K)
ΔH° :n/a
ΔHobs :-26±0.5 (kcal/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:no
Protons Released:n/a
ΔCp :n/a
Stoich. Param.:0.86
ΔS° :n/a
Comments:n/a
 
Citation Chilkoti, ABoyer, FEStayton, PSVara Prasad, JVDomagala, JMEllsworth, ELGajda, CHagen, SEMarkoski, LJTait, BDLunney, EAPalovsky, AFerguson, DGraham, NHoller, THupe, DNouhan, CTummino, PJUrumov, AZeikus, EZeikus, GGracheck, SJSanders, JMVanderRoest, SBrodfuehrer, JIyer, KSinz, MGulnik, SV  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Data Fit Method ,  Instrument Info
 
Streptavadin(W79F)
Source:Mutant protein was expressed in a T7 expression system (pET-21a, Novagen Inc.,Madison,WI) as insoluble inclusion bodies.
Purity:n/a
Prep. Method:Refolded protein was purified by affinity chromatography with iminobiotin-agarose.
Name:Streptavadin(W79F)
Synonyms:n/a
Type:Biotin-Binding Protein
Mol. Mass.:18796.50
Organism:Streptomyces avidinii
Description:n/a
Residue:183
Sequence:
MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGAD
GALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAFKNNYRNAHSATTWSGQY
VGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDA
VQQ
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM12
Source:n/a
Purity:n/a
Prep. Method:n/a
NameBDBM12
Synonyms:5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoic acid | Biotin | cid_171548 | cid_253
TypeEnzyme co-factor
Emp. Form.C10H16N2O3S
Mol. Mass.244.311
SMILES[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2 |r|
Structure
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