Reaction Details 
Report a problem with these data
Report a problem with these dataCell Reactant:
Major Urinary Protein (MUP-1)
Syringe Reactant:
BDBM22606
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
06/15/08
ΔG°:
-35.6±0.1 (kJ/mole)
pH:
7.4±n/a
Log10Kb:
5.2
Temperature:
300±n/a (K)
ΔHobs :
-58±0.6 (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
1.05
ΔS° :
-0.0747±0.002 (kJ/mole-K)
Comments:
Kd value was obtained from J. Mol. Biol. (2006) 362,994-1003.
Citation
Malham, R; Johnstone, S; Bingham, RJ; Barratt, E; Phillips, SE; Laughton, CA; Homans, SW Strong solute-solute dispersive interactions in a protein-ligand complex. J Am Chem Soc 127:17061-7 (2005) [PubMed] ArticleCell React
Source:
The MUP-1 gene was cloned and expressed in E. coli cells.
Prep. Method:
MUP-I was purified using Ni-NTA resin. To remove endogenous bound ligands, an ethanol precipitation step was utilized involving addition of 2 volumes of absolute ethanol per volume of protein solution, followed by incubation at 4 °C for 2 h. After centrifugation at 5000g, the pellet was lyophilised, resuspended in PBS pH 7.4, and dialyzed overnight against the same buffer.
Name:
Major Urinary Protein (MUP-1)
Synonyms:
MUP1_MOUSE | Major urinary protein 1 precursor | Mup1
Type:
Other Protein Type
Mol. Mass.:
20640.17
Organism:
Mus musculus (mouse)
Description:
n/a
Residue:
180
Sequence:
MKMLLLLCLGLTLVCVHAEEASSTGRNFNVEKINGEWHTIILASDKREKIEDNGNFRLFLEQIHVLENSLVLKFHTVRDEECSELSMVADKTEKAGEYSVTYDGFNTFTIPKTDYDNFLMAHLINEKDGETFQLMGLYGREPDLSSDIKERFAQLCEKHGILRENIIDLSNANRCLQARE
Syringe React
Name:
BDBM22606
Synonyms:
1-octanol | Octan-1-ol | n-Octyl Alcohol
Type:
Small organic molecule
Emp. Form.:
C8H18O
Mol. Mass.:
130.2279
SMILES:
CCCCCCCCO
Structure:
