Cell Reactant:

Syringe Reactant:

Meas. Tech.:

ITC

Entry Date.:

12/04/00

ΔG°:

-46.2±0.84 (kJ/mole)

pH:

8.5±n/a

Log₁₀Kb:

8

Temperature:

298.15±n/a (K)

ΔH° :

-71.4±n/a (kJ/mole)

ΔH_obs :

-71.4±n/a (kJ/mole)

ΔS° :

-0.0798±0.04 (kJ/mole-K)

Comments:

binding is very tight, limiting the accuracy of calorimetric measurement of the binding constant to approximately 25%

Citation

 Robinson, CR; Liu, Y; Thomson, JA; Sturtevant, JM; Sligar, SG Energetics of heme binding to native and denatured states of cytochrome b562. Biochemistry 36:16141-6 (1997) [PubMed]  Article

More Info.:

Get all data from this article,  ITC RUN data,  Solution Info,  Instrument Info

Source:

E.coli

Prep. Method:

Holoprotein was purified by ion-exchange FPLC just prior to heme extraction

Name:

apocytochrome b562

Synonyms:

C562_ECOLX | apocytochrome b562(denatured) | cybC | denatured apocytochrome b562

Type:

Protein

Mol. Mass.:

14060.82

Organism:

Escherichia coli

Description:

P0ABE7

Residue:

128

Sequence:

MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR

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Blast E-value cutoff:

Source:

Sigma Chemical Co.

Prep. Method:

dissolved in NaOH and diluted with buffer

Name:

BDBM20

Synonyms:

3-[5-(2-carboxyethyl)-15,20-diethenyl-22-hydroxy-4,10,14,19,24-pentamethyl-21,23,24,25-tetraaza-22-ferrahexacyclo[9.9.3.1^{3,6}.1^{13,16}.0^{8,23}.0^{18,21}]pentacosa-1(20),3,5,8,10,13,15,18-octaen-9-yl]propanoic acid | Hematin(iron protoporphyrin IX)

Type:

Porphyrin

Emp. Form.:

C35H40N4O4

Mol. Mass.:

580.7176

SMILES:

Cc1c2Cc3[n-]c(Cc4c(C=C)c(C)c(Cc5[n-]c(Cc([nH]2)c1CCC(O)=O)c(CCC(O)=O)c5C)n4C)c(C)c3C=C

Structure:

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