Reaction Details  Report a problem with these data
Cell Reactant:
Transcriptional Regulator TtgR
Syringe Reactant:
BDBM19461
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
07/23/08
ΔG°:
-24.2858±0.1672 (kJ/mole)
pH:
7±n/a
Log10Kb:
3
Temperature:
303.15±n/a (K)
ΔHobs :
-14.421±2.09 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
0.0325±0.007 (kJ/mole-K)
Citation
 Terán, W; Krell, T; Ramos, JL; Gallegos, MT Effector-repressor interactions, binding of a single effector molecule to the operator-bound TtgR homodimer mediates derepression. J Biol Chem 281:7102-9 (2006) [PubMed]  Article
Cell React
Source:
A 651-bp fragment containing the ttgR gene was amplified by PCR from P. putida DOT-T1E chromosomal DNA, and cloned in E. coli expression vector. TtgR protein was over-expressed and purified.
Prep. Method:
TtgR protein was further purified by size exclusion chromatography using a Sephacryl HR-200 column (Amersham Biosciences). Eluted fractions of TtgR were pooled, concentrated, and dialyzed against the buffer.
Name:
Transcriptional Regulator TtgR
Synonyms:
HTH-type transcriptional regulator ttgR | TTGR_PSEPT | Toluene efflux pump ttgABC operon repressor | ttgR
Type:
Repressor; homodimer
Mol. Mass.:
23852.57
Organism:
Pseudomonas putida
Description:
n/a
Residue:
210
Sequence:
MVRRTKEEAQETRAQIIEAAERAFYKRGVARTTLADIAELAGVTRGAIYWHFNNKAELVQALLDSLHETHDHLARASESEDEVDPLGCMRKLLLQVFNELVLDARTRRINEILHHKCEFTDDMCEIRQQRQSAVLDCHKGITLALANAVRRGQLPGELDAERAAVAMFAYVDGLIRRWLLLPDSVDLLGDVEKWVDTGLDMLRLSPALRK
  
Syringe React
Prep. Method:
All chemicals were manipulated in glass vessels and effector samples were neither degassed nor filtered, to avoid evaporation or nonspecific binding. Analyses were carried out using 5% DMSO to facilitate solubilization.
Name:
BDBM19461
Synonyms:
α-CA inhibitor, 5 | 5,7-dihydroxy-2-(4-hydroxyphenyl)-3,4-dihydro-2H-1-benzopyran-4-one | CHEMBL32571 | Naringenin | Naringenin (NAR) | naringetol | salipurpol
Type:
Estrgen Antagonist
Emp. Form.:
C15H12O5
Mol. Mass.:
272.2528
SMILES:
Oc1ccc(cc1)C1CC(=O)c2c(O)cc(O)cc2O1
Structure:
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