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Cell Reactant:
Transcriptional Regulator TtgR Mutant R176G
Syringe Reactant:
BDBM23446
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
07/23/08
ΔG°:
-29.26±0.418 (kJ/mole)
pH:
7±n/a
Log10Kb:
3.8
Temperature:
303.15±n/a (K)
ΔHobs :
-90.288±2.09 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
-0.2013±0.0028 (kJ/mole-K)
Citation
Alguel, Y; De Lucca, GV; Liang, J; Meng, C; Terán, W; De Lucca, I; Krell, T; Ramos, JL; Gallegos, MT; Zhang, X Crystal structures of multidrug binding protein TtgR in complex with antibiotics and plant antimicrobials. J Mol Biol 369:829-40 (2007) [PubMed] Article
Cell React
Source:
Site-directed mutant was cloned, and protein was expressed and purified from E. coli.
Prep. Method:
TtgR mutant protein was further purified by size exclusion chromatography using a Sephacryl HR-200 column (Amersham Biosciences). Eluted fractions of TtgR were pooled, concentrated, and dialyzed against the buffer.
Name:
Transcriptional Regulator TtgR Mutant R176G
Synonyms:
HTH-type transcriptional regulator ttgR | Toluene efflux pump ttgABC operon repressor
Type:
Repressor; homodimer
Mol. Mass.:
23752.43
Organism:
Pseudomonas putida
Description:
n/a
Residue:
210
Sequence:
MVRRTKEEAQETRAQIIEAAERAFYKRGVARTTLADIAELAGVTRGAIYWHFNNKAELVQALLDSLHETHDHLARASESEDEVDPLGCMRKLLLQVFNELVLDARTRRINEILHHKCEFTDDMCEIRQQRQSAVLDCHKGITLALANAVRRGQLPGELDAERAAVAMFAYVDGLIGRWLLLPDSVDLLGDVEKWVDTGLDMLRLSPALRK